Project description:Genetically engineering Nicotiana tabacum to express Isoprene Synthase (ISPS) leads to changes in expression of genes assoiated with many growth regulator signaling pathways and signaling networks involved in abiotic and biotic stress responses.
Project description:The study aims to identify and analyze the miRNAs in Bemisia tabaci that feed on Nicotiana tabacum cv. NC89 plants. This allows for the investigation of insect miRNAs that play important physiological roles in Bemisia tabaci. Additionally, it enables the analysis and identification of miRNAs that are transferred from Nicotiana tabacum to Bemisia tabaci. These data can further help us understand the role of miRNAs in the interaction between Bemisia tabaci and its host plants.
Project description:Transcriptome profiling of three developmental stages of immature male gametophyte intobacco (Nicotiana tabacum) Total RNA isolated from tobacco microspores and early and late bicellular pollen was hybridised on Agilent Tobacco Gene Expression Microarray 4x44K in two biological replicates per sample
Project description:Along with lipidomic and metabolomic analyses, we analysed the effect of short-term heat stress on Nicotiana tabacum pollen tubes. Tubes were either grown for 3 hours at room temperature, for 6 hours at room temperature or for 3 hours at room temperature and then 37 °C for another 3 hours.