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Chlamydomonas reinhardtii

ABSTRACT: Global Changes following N-deprivation in Chlamydomonas: Illumina sequencing

PROVIDER: PRJNA133477 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR066643.fastq.gz	Fastqsanger.gz
	SRR066644.fastq.gz	Fastqsanger.gz
	SRR066645.fastq.gz	Fastqsanger.gz
	SRR066646.fastq.gz	Fastqsanger.gz
	SRR066647.fastq.gz	Fastqsanger.gz

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Global Changes following N-deprivation in Chlamydomonas

Project description:This SuperSeries is composed of the following subset Series: GSE24365: Global Changes following N-deprivation in Chlamydomonas: Illumina sequencing GSE24366: Global Changes following N-deprivation in Chlamydomonas: 454 sequencing Refer to individual Series

2010-09-24 | E-GEOD-24367 | biostudies-arrayexpress

Chlamydomonas reinhardtii

Project description:Global Changes following N-deprivation in Chlamydomonas: 454 sequencing

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RNA-seq of Chlamydomonas reinhardtii response to nitrogen deprivation

Project description:Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient-deprived. To begin studying the mechanisms underlying this process, N-deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparative global analysis of transcripts under induced and non-induced conditions was applied as a first approach to study molecular changes that promote or accompany triacylglycerol accumulation in cells encountering a new nutrient environment. Towards this goal, high-throughput sequencing technology was employed to generate large numbers of expressed sequence tags of 8 biologically independent libraries (6 Illumina libraries, 2 454 libraries), four for each condition, N-replete and N-deprived, that allowed a statistically sound comparison of expression levels under the two tested conditions. Inferences on metabolism based on transcriptional analysis can only be indirect but were supported in parts by biochemical experiments. N-deprivation led to a marked redirection of metabolism as the carbon source acetate was no longer converted to cell building blocks by the glyoxylate cycle and gluconeogensis, but funneled directly into fatty acid biosynthesis. Protein biosynthesis and photosynthesis were down-regulated and genes of gametogenesis activated. A notable finding was that the genes encoding photosynthetic accessory PSBS proteins of currently unclear function in C. reinhardtii were strongly expressed following N-deprivation, providing a clue to their physiological role. Lipase-encoding genes were found to be some of the most regulated following N-deprivation, suggesting a remodeling of membranes under these conditions. The data provided here represent a rich source for the exploration of the mechanism of oil accumulation in microalgae. Examining N-replete and N-deprived conditions. Three biological replicates each condition.

2010-09-24 | E-GEOD-24365 | biostudies-arrayexpress

Chlamydomonas reinhardtii strain:dw15

Project description:Global Changes following N-deprivation and N-resupply in Chlamydomonas in the cht7 mutant and the wild-type: Illumina sequencing

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Global Changes following N-deprivation in Chlamydomonas

Project description:Global Changes following N-deprivation in Chlamydomonas

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UV-B-induced gene expression changes in Chlamydomonas reinhardtii

Project description:We analysed global gene expression changes in Chlamydomonas reinhardtii in response to 1h UV-B, applied at the same low level that was seen to promote subsequent UV-B stress tolerance, in order to elucidate the transcriptional reprogramming that leads to UV-B acclimation. mRNA profiles generated by deep sequencing from triplicate replicate Chlamydomonas reinhardtii samples sourced from independent cultures either protected from UV-B or exposed to 1h acclimation-level UV-B.

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2007-05-15 | E-GEOD-7308 | biostudies-arrayexpress

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