Project description:Transcriptomic analysis was performed in Deinococcus geothermalis wild-type and three mutants including dgeo_0257, dgeo_0281, and double knockout (DK) mutants. dgeo_0281 is a major Dps-coding gene and dgeo_0257 is a novel Dps candidate. Thus, to detecte physiological functions of both Dps-encoded genes, each gene-disrupted mutant and DK mutant was constructed and performed RNA-Seq analysis. This result support better understanding for Dps roles in radiation resistant bacterium through up-regulated and down-regulated genes profile.
Project description:We isolated an aerobic autotrophic bacterium from a hot spring on Kunashir Island and identified it as Thermus brevis strain PS18 cells were short straight nonmotile rods, 0.5x1.0-1-2 mkm. Strain PS18 grows autotrophically aerobically with thiosulfate (electron donor) and O₂ (acceptor). Autotrophy has been previously unknown in representatives of the Deinococcota phylum. Autotrophic growth of PS18 under aerobic and anaerobic conditions was characterized in growth experiments by proteomic analyses.
