Staphylococcus aureus
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ABSTRACT:
PROVIDER: PRJNA135087 | ENA |
REPOSITORIES: ENA
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Project description:The study aims to compare gene expression patterns of Staphyloccoccus aureus USA300 wild type (reference) vs USA300 agr knockout(query). The agr quorum sensing system is an important regulator of density dependent gene expression. To determine the role of agr in USA300 virulence a agr mutant was constructed and differential gene expression compared to the parental wildtype.
2011-04-08 | GSE26254 | GEO
Project description:Compilation fo whole genome gene expression changes in Staphylococcus aureus USA300 LAC cultures grown in the presence of vehicle or the anti-gout drug benzbromarone. The drug was intially screened as effective against the agr quorum sensing system in Staphylococcus aureus AH1677. A microarray study using total RNA harvested from three cultures of Staphylococcus aureus USA300 LAC plus vehicle control and three cultures of Staphylococcus aureus USA300 LAC plus 12 uM benzbromarone.
2014-03-19 | E-GEOD-55980 | biostudies-arrayexpress
Project description:Compilation fo whole genome gene expression changes in Staphylococcus aureus USA300 LAC cultures grown in the presence of vehicle or the anti-gout drug benzbromarone. The drug was intially screened as effective against the agr quorum sensing system in Staphylococcus aureus AH1677.
2014-03-19 | GSE55980 | GEO
Project description:Gene expression analysis of Staphyloccoccus aureus USA300 vs USA300 sae knockout
| PRJNA135071 | ENA
Project description:The study aims to compare gene expression patterns of Staphyloccoccus aureus USA300 vs USA300 sae knockout. Samples were hybridized on aminosilane coated slides with 70-mer oligos.
2011-04-08 | GSE26243 | GEO
Project description:Gene expression analysis of Staphyloccoccus aureus USA300 vs USA400.
| PRJNA135089 | ENA
Project description:CodY is a conserved regulator in gram-positive organisms described to repress metabolic genes mainly involved in nitrogen metabolism but also to control the expression of virulence genes in pathogens. We constructed codY gene-replacement mutants in three unrelated S. aureus strains (Newman, UAMS-1, RN1HG). codY mutants grew slower in a chemically defined medium compared to the wild type strains. However, only codY mutants were able to grow in medium lacking threonine. Excess of isoleucine resulted in growth inhibition in the wild type but not in the codY mutants indicating a role of isoleucine for CodY dependent repression of metabolic genes. The prototypic CodY-repressed operon ilvDBCleuABCilvA is preceded by a CodY-binding motif and repressed after up-shift with isoleucine and to a lesser extent guanidine. Transcription of the quorum sensing system agr followed a similar expression pattern. The codY dependent repression of agr is in line with the concomitant influence of CodY on typical agr regulated genes such as cap, spa fnbA and coa. However, transcriptional analysis revealed that most of these virulence genes (e.g. cap, fnbA, spa hla) are also regulated by CodY in an agr negative background. Microarray analysis revealed the large majority of codY-repressed genes are involved in amino-acid transport and metabolism, genes showing codY dependent activation were mainly involved in nucleotide transport and metabolism or virulence. In summary, CodY in S. aureus not only acts as repressor for genes involved in nitrogen metabolism but also contributes to virulence gene regulation by supporting as well as substituting agr function. The microarray was manufactured by in situ synthesis of 10'807 long oligonucleotide probes , selected as previously described (Charbonnier, 2005). It covers >98% of all ORFs annotated in strains N315 and Mu50 , MW2, COL , NCTC8325, USA300 , MRSA252 an MSSA476 including their respective plasmids.
2008-11-06 | E-GEOD-12340 | biostudies-arrayexpress
Project description:Staphylococcus aureus (S. aureus) is a formidable human pathogen that uses secreted cytolytic factors such as hemolysins, leukocidins and cytolytic peptides to injure immune cells and promote infection of its host. Of these secreted molecules, the bicomponent family of pore-forming leukocidins are a potent class of cytolytic proteins with critical contributions to S. aureus pathogenesis. The regulatory mechanisms governing the expression of these toxins are incompletely defined. In this work, we performed a screen to identify transcriptional regulators involved in leukocidin expression in S. aureus USA300. We discovered that a metabolic sensor regulator, RpiRc, is a potent and selective repressor of two leukocidins, LukED and LukSF-PV. Whole genome transcriptomics, S. aureus exoprotein proteomics and metabolomic analyses revealed that RpiRc influences the expression and production of disparate virulence factors, in addition to altering metabolic fluxes in the TCA cycle, glycolysis and amino acid metabolism. Using mutational analyses, we confirmed that RpiRc signals through the accessory gene regulatory quorum-sensing system (Agr) in USA300. Specifically, in wildtype USA300, RpiRc represses the rnaIII promoter resulting in increased production of the repressor of toxins –Rot, which in turn represses leukocidin expression. In fact, deletion of rpiRc phenocopied deletion of rot. We show that RpiRc-mediated regulation of virulence factors is critical for killing of primary human polymorphonuclear leukocytes and for the pathogenesis of bloodstream infection in vivo. Collectively, our results suggest that S. aureus senses metabolic shifts by RpiRc to differentially regulate the expression of leukocidins thereby promoting invasive disease.
2017-01-26 | GSE77301 | GEO
Project description:The study aims to compare gene expression patterns of Staphyloccoccus aureus USA300 vs USA300 sae knockout. Samples were hybridized on aminosilane coated slides with 70-mer oligos. 12 arrays total.
2011-04-08 | E-GEOD-26243 | biostudies-arrayexpress
Project description:In the community and clinical settings MRSA infections are more commonly caused by a USA300 strain vs a USA400 strain. The study aims to compare gene expression patterns of Staphyloccoccus aureus USA300(reference) vs USA400(query) and to determine why USA400 is being replaced by a USA300 strain.
2011-04-08 | GSE26255 | GEO