Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA136019 | ENA |
REPOSITORIES: ENA
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Project description:Vascular hypoperfusion is a pathological phenomenon in the glaucomatous optic nerve head. We report transcriptional responses in GFAP-negative LC cells exposed to in-vitro hypoxic stress (1%O2). The study design was as follows: For GSM659595: "human donor 1" LC cells were passaged into three p100 plates of cells and then exposed to normoxia for 24 hours. The RNA from each of these three plates was then extracted, pooled and hybridised to the microarray "normoxia_1". For GSM659592: "human donor 1" LC cells were passaged into three p100 plates of cells and then exposed to hypoxia for 24 hours. The RNA from each of these three plates was then extracted, pooled and hybridised to the microarray "hypoxia_1". "hypoxia_1" and "normoxia_1" microarray data were then compared to calculate differential gene expression in response to hypoxia. This experiment (same design) was then conducted a second and third time using biologically independent "human donor 2" and "human donor 3" cell lines respectively.
2012-03-01 | E-GEOD-26820 | biostudies-arrayexpress
Project description:Vascular hypoperfusion is a pathological phenomenon in the glaucomatous optic nerve head. We report transcriptional responses in GFAP-negative LC cells exposed to in-vitro hypoxic stress (1%O2).
2012-03-01 | GSE26820 | GEO
Project description:Purpose: Marked extracellular matrix (ECM) remodeling occurs in the human optic nerve head in primary open angle glaucoma (POAG). The glial fibrillary acid protein (GFAP) negative lamina cribrosa cell may play an important role in this remodeling process. The authors report the first study of global and ECM-focused gene transcription differentials between GFAP-negative negative lamina cribrosa (LC) cells from normal and POAG human donors. Methods: GFAP-negative LC cell lines were generated from the optic nerve tissue of three normal (n=3) and three POAG (n=3) human donors. Using Affymetrix U133A arrays the transcriptional profile between the normal and diseased groups were compared. Bioinformatic analysis was carried out using robust multichip average (RMA Express) and EASE/David. Real time TaqMan PCR and immunohistochemistry analyses were performed to validate the microarray data. Results: 285 genes were up regulated by greater than 1.5 fold and 413 were down regulated by greater than 1.5 fold in the POAG LC cells versus normal controls. Upregulated genes in POAG LC cells included, SPARC, periostin, thrombospondin, CRTL-1, CTGF and collagen types I, III, V and VIII. Downregulated ECM genes in POAG included MMP-1, fibulin, decorin and tenacsin XB. All TaqMan PCR validation assays were significant (*p<0.05) and consistent with the array data. Immunohistochemistry of one target (periostin) confirmed its differential expression at the protein level in POAG optic nerve head tissue compared with non-glaucomatous controls. Functional annotation and over-representation analysis identified ECM genes as a statistically over-represented class of genes in POAG LC cells compared with normal LC cells. Conclusions: This study reports for the first time that POAG LC cells in-vitro demonstrate up regulated ECM and pro-fibrotic gene expression compared with normal LC cells. This may be a pathological characteristic of this cell type in POAG in-vivo. We believe that the LC cell may be a pivotal regulator of optic nerve head ECM remodeling and an attractive target for future therapeutic strategies in POAG.
2009-01-06 | GSE13534 | GEO
Project description:To assess the transcriptomic response of the LUAD cell line A549 to hypoxia, cells were exposed to 1% (Hx) or 21% O2 (Nx) during 24 hours. Two independent experiments were carried out.
2019-09-04 | GSE117041 | GEO
Project description:Three independent parental or NLUCAT1 CRISPR/Cas9-deleted A549 clones were cultured in normoxia (Nx) or exposed to hypoxia (Hx) at 1% O2 for 24 hours.
2019-09-04 | GSE117036 | GEO
Project description:An iTRQA-based quantitative proteome analysis using liquid chromatography–coupled tandem mass spectrometry was performed to acquire proteome-wide expression data on Triple-negative Breast Cancer (TNBC) cells treated with Rocaglamide A for 24 hours.
2024-12-08 | PXD047648 | JPOST Repository
Project description:Expression data from hypoxia exposed normal human esophageal epithelial cells
| PRJNA119069 | ENA
Project description:We hypothesize that the culture media collected from macrophages exposed to intermittent hypoxia will induce a greater pro-inflammatory gene profile in naïve cultured macrophages than will culture media collected from macrophages exposed to sustained hypoxia. We will evaluate gene expression using microarray analysis of RNA collected from RAW 264.7 macrophages cultured for 24 hours in DMEM media obtained from 1) cells cultured with intermittent hypoxia (2 minute cycles: 90 seconds at 40 Torr and 30 seconds at 8 Torr), 2) media exposed to intermittent hypoxia, 3) cells cultured with sustained hypoxia (8 Torr), 4) media exposed to sustained hypoxia and 4) standard tissue culture conditions (fresh DMEM media; reference).
2005-01-01 | E-TIGR-91 | biostudies-arrayexpress
Project description:To determine if the changes in MHC I expression under hyppoxia results in a change in the antigen presentation, we used LC-MS technology to analyze the immunopeptidome. HCT116 cells were treated either in normoxia (21% O2) or hypoxia for 24 hours cells were then collected and a quantitative analysis of the immunopeptidome was performed
2024-11-04 | PXD055205 | Pride