Project description:Mouse and human somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by the transduction of four transcription factors, Oct 3/4, Sox2, Klf4, and c-Myc. Conducting reprogramming in hypoxic conditions was shown to result in improved efficiency for both mouse and human cells. To examine the effect of hypoxic cultivation, we performed microarray analyses using mouse embryonic fibroblasts cultivated under normoxia and hypoxia.
Project description:Transcription profiling by high throughput sequencing of mouse embryonic fibroblasts, terminally differentiated neurons and embryonic stem cells
Project description:Gene expression in human umbilical cord vein and artery endothelial cells under physiological chronic normoxia (3% O2, PCN) and standard culture normoxia (21% O2, SCN)
