Schizophyllum commune
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ABSTRACT:
PROVIDER: PRJNA136853 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The transcription factors Hom2 and Fst4 were shown to be involved in mushroom development in the basidiomycete Schizophyllum commune. When the genes encoding Hom2 or Fst4 were deleted, no mushrooms formed. In this study we performed a genome-wide expression analysis on dikaryons of wild-type, delta-hom2 and delta-fst4.
2012-01-20 | GSE23594 | GEO
Project description:Transcriptome changes associated with mating interactions were performed in order to identify signaling components and targets of pheromone response. Two monokaryons (S. commune 12-43 and 4-39), a dikaryon (W22 x 12-43), both semi-compatible mating interactions (Aon: W22 x 4-39; Bon, flat: W21 x 4-39), and different S. commune pheromone receptor recipient strains (Vbar2f, Vbar2t) were analyzed for that purpose. In addition, a S. commune strain showing the thin-phenotype (W22-thin) was investigated to figure out the role of Thn1 in mating. With microarray analysis mRNA expression in different mating interactions of the basidiomycete S. commune was analyzed. Genes which show regulation in the comparison of the two monokaryons 12-43 and 4-39 has been eliminated from other comparisons as strain specific differences. Two biological or technical replicate samples were analyzed for each condition.
2012-06-14 | E-GEOD-26401 | biostudies-arrayexpress
Project description:In order to study the effect of light on gene expression in Schizophyllum commune, genome wide gene expression was analysed in 4-day-old monokaryotic and dikaryotic wild type colonies, grown either in light or in darkness.
2013-08-12 | GSE23533 | GEO
Project description:The transcription factors Hom2 and Fst4 were shown to be involved in mushroom development in the basidiomycete Schizophyllum commune. When the genes encoding Hom2 or Fst4 were deleted, no mushrooms formed. In this study we performed a genome-wide expression analysis on dikaryons of wild-type, delta-hom2 and delta-fst4. A total of three samples were analyzed. One wild-type and two deletion strains (delta-hom2 and delta-fst4)
2012-01-20 | E-GEOD-23594 | biostudies-arrayexpress
Project description:Transcriptome changes associated with mating interactions were performed in order to identify signaling components and targets of pheromone response. Two monokaryons (S. commune 12-43 and 4-39), a dikaryon (W22 x 12-43), both semi-compatible mating interactions (Aon: W22 x 4-39; Bon, flat: W21 x 4-39), and different S. commune pheromone receptor recipient strains (Vbar2f, Vbar2t) were analyzed for that purpose. In addition, a S. commune strain showing the thin-phenotype (W22-thin) was investigated to figure out the role of Thn1 in mating.
2012-06-15 | GSE26401 | GEO
Project description:schizophyllum commune population study Genome sequencing
| PRJNA236351 | ENA
Project description:Intracellular signaling is conserved in eukaryotes to allow for response to extracellular signals and to regulate development and cellular functions. In fungi, inositol phosphate signaling has been shown to be involved in growth, sexual reproduction, and metabolic adaptation lacking, however, reports of mushroom forming fungi so far. In Schizophyllum commune, an inositol monophosphatase has been found up-regulated during sexual development. The enzyme is crucial for inositol cycling where it catalyzes the last step of inositol phosphate metabolism restoring the inositol pool from the monophosphorylated inositol monophosphate. We overexpressed the gene in this model basidiomycete and verified its involvement in cell wall integrity and intracellular trafficking. Strong phenotypes in mushroom formation and cell metabolism were evidenced by proteome analyses. In addition, altered inositol signaling was shown to be involved in tolerance towards cesium and zinc, and increased metal tolerance towards cadmium, associated with induced expression of kinases and repression of phosphatases within the inositol cycle. The presence of the heavy metals Sr, Cs, Cd, and Zn lowered intracellular calcium levels. We could develop a model integrating inositol signaling in the known signal transduction pathways governed by Ras, G-protein coupled receptors, cAMP and elucidate different roles in development.
2021-07-01 | PXD026465 | Pride
Project description:Schizophyllum commune is a wood decay fungus known for its antioxidant, anti-tumor, immunological, and antimicrobial properties. Nonetheless, the effects of growth conditions on the activation of metabolic pathways are still poorly understood for this fungus. To this aim, proteomics is one of the most powerful approaches in medicine and biology for the characterization of molecular pathways, protein regulation, and protein-protein interactions of living organisms. A crucial aspect of the proteomics pipeline is the protein extraction method, since it strongly influences the information gained on molecular processes and the understanding of underlying biological mechanisms. In this work, Schizophyllum commune was employed as a model fungus to compare the efficiency of two popular protein extraction methods and to analyze the proteomic profile of mycelium grown in liquid medium. A strain of Schizophyllum commune preserved in the Fungal Research Culture Collection of the University of Pavia (MicUNIPV) was grown on a culture medium composed of malt extract and its protein content was assessed by two distinct extraction methods followed by mass spectrometry analyses. The results show that the protocol based on the combined use of Tris-Cl and Urea led to a significantly higher extraction efficiency, both in terms of the number of proteins identified and the variety of the cellular compartments characterized. Furthermore, functional analysis on identified proteins shows that specific metabolic processes are activated in the growth phase under investigation: catabolism and anabolism of proteins and polysaccharides, pentose phosphate shunt, tricarboxylic acid cycle, and oxidative stress response. Altogether, these results show how to optimize the investigation of the proteomic profiles of Schizophyllum commune, and how this approach would lead to an expansion of its potential industrial applications.
2025-05-06 | PXD048036 | Pride
Project description:In order to study the effect of light on gene expression in Schizophyllum commune, genome wide gene expression was analysed in 4-day-old monokaryotic and dikaryotic wild type colonies, grown either in light or in darkness. 4 samples: - monokaryon, grown for 4 days in the light - monokaryon, grown for 4 days in the dark - dikaryon, grown for 4 days in the light - dikaryon, grown for 4 days in the dark RNA was obtained from 3 biological replicates and pooled
2013-08-12 | E-GEOD-23533 | biostudies-arrayexpress
Project description:The blue light receptor WC-2 was shown to be involved in mushroom development in the basidiomycete Schizophyllum commune. When the gene encoding WC-2 was deleted, no mushrooms formed and colony morphology was radial. This phenotype was similar to the wild-type colony grown in the dark. This phenotype could be complemented by transforming the wc-2 deletion strain with a construct encompassing the wc-2 coding sequence under the control of the heat inducible promoter hsp3. A daily heat shock of 1 hour at 42 degrees Celsius resulted in mushroom development and an asymmetrical colony. In this study we performed a genome-wide expression analysis on dikaryons of wild-type (not heat shocked), delta-wc2 (heat shocked or not heat shocked) and the complemented strain delta-wc2 hsp3-wc2 (heat shocked or not heat shocked).
2012-09-24 | GSE24007 | GEO