Project description:Transcription factor pancreatic and duodenal homeobox 1 (Pdx1) plays an essential role in the pancreas to regulate its development and maintain proper islet function. However, less is known about the function of Pdx1 in the small intestine. We aim to investigate the role of Pdx1 in mature proximal small intestine by profiling the expression of genes differentially regulated in response to Pdx1 inactivation restricted to the intestinal epithelium in mice. Pdx1 was conditionally inactivated in the intestinal epithelium of Pdx1flox/flox;VilCre mice, by crossing mutant mice homozygous for loxP site-flanked Pdx1 alleles with transgenic mice expressing Cre recombinase under the control of the mouse villin 1 gene promoter. Total RNA was isolated from the first five centimeters of the small intestine from adult Pdx1flox/flox;VilCre and littermate control mice. Microarray analysis was performed to investigate genome-wide transcriptional profiles in the proximal small intestine.

2012-01-12 | E-GEOD-29048 | biostudies-arrayexpress

E-MTAB-3139

Project description:Transcriptome profiling of mouse pancreatic epithelium from Pdx1-Ngn3ER-IRES-GFP mice bred into a Neurog3-null background at embryonic day 11.25

2017-02-09 | E-MTAB-3139 | ExpressionAtlas

Mus musculus

Project description:Transcript Profiling of Elf5+/- Mammary Glands During Pregnancy Identifies Novel Targets of Elf5

| PRJNA131271 | ENA

E-GEOD-29364

Project description:Transcription profiling of Arabidopsis WUSCHEL targets

2015-12-02 | E-GEOD-29364 | ExpressionAtlas

Pdx1 overexpression sensitizes to IL-1b-induced apoptosis

Project description:Cytokines have been shown to play a key role in the destruction of beta cells. In the rat insulinoma cell line (INS-1ab) overexpressing pancreatic duodenum homeobox 1 (Pdx1) increases sensitivity to Interleukin 1b (IL-1b). To elucidate mechanisms of action underlying Pdx1 driven potentiation of beta-cell sensitivity to IL-1β, we performed a microarray analysis of INS-1ab cells with and without Pdx1 overexpression exposed to IL-1β between 2h and 24h.

2012-09-20 | GSE40642 | GEO

E-MTAB-964

Project description:Transcription profiling by high throughput sequencing of maize mature silk, mature pollen, mature ovary, and seedlings

2016-10-06 | E-MTAB-964 | ExpressionAtlas