Project description:Transcriptional profiling of M. smegmatis and Dcrp1 grown in flasks on Hartmans de Bont medium supplemented with 10 mM glycerol. Transcriptional profiling of M.smegmatis HLA102 harboring tetracycline inducible vector pMind containing the Crp2 and M. smegmatis harboring pMind grown in flasks on Hartmans de Bont medium supplemented with 10 mM glycerol
Project description:Transcriptional profiling of M. smegmatis and Dcrp1 grown in flasks on Hartmans de Bont medium supplemented with 10 mM glycerol. Transcriptional profiling of M.smegmatis HLA102 harboring tetracycline inducible vector pMind containing the Crp2 and M. smegmatis harboring pMind grown in flasks on Hartmans de Bont medium supplemented with 10 mM glycerol Comparing transcriptional response of wild-type compared with the deletion of crp1 gene (Msmeg_0539). Biological replicates: 4 independently grown and harvested. One replicate per array. Comparing transcriptional response of strain HLA102 to conditional expression of Crp2 (Msmeg_6189) compared with the wild-type with empty vector pMind. Biological replicates: 4 independently grown and harvested. One replicate per array.
Project description:RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.
Project description:Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage in Darr strains expressing ArrWT, ArrH18A, ArrD83A, or an empty vector vs WT M. smegmatis Mc2155 (mgm182, site(+))
