Project description:Transcripts for GH, MHC Class I and II genes, and heavy- and light-chain myosins, as well as many others genes, were differentially regulated in the zebrafish inner ear following overexposure to sound. Following acoustic trauma in the zebrafish inner ear, we used microarray analysis to identify genes involved in inner ear repair following acoustic exposure by comparing the gene expression levels of 2 days and 4 days post-sound exposure (NE-ZF-2d and NE-ZF-4d, respectively) to controls without sound exposure (C-ZF).
Project description:Hearing functions through the mechanical transduction of inner ear sensory cells, hair cells, and their connections to the auditory neurons, which convert the stimuli into electrical signals to be detected by the brain. Noise-induced hearing loss (NIHL) caused by exposure to excessively sounds cannot be medically corrected. Strategies to overcome the apparently irreversible noise-induced hearing loss (NIHL) in mammals become paramount for hearing treatment. The drug has been reported in attenuating inflammation in different species. Here, we tested the effect of the drug in acoustic trauma. We carried out single-cell RNA sequencing to examine the gene expression profiles comparing Drug group with Untreated group in response to the acoustic trauma.
Project description:RNAseq was performed on hcar1-4+/+ and hcar1-4-/- zebrafish larvae with (2 h and 4 h) or without (0 h) Pseudomonas aeruginosa (PA) ear infection. The transcriptome profile generated here reveals PA14 infection-induced differential gene expression patterns between hcar1-4-/- and hcar1-4+/+ siblings.
