Project description:In order to elucidate the role of the Arabidopsis thaliana LLM-domain B-GATAs in response to high light intensities, a transcriptomic analysis of Col-0, a hexuple LLM-domain B-GATA mutant hex (gnc gnl gata15 gata16 gata17 gata17l) and GNLox under high-ligh stress conditions was performed.
Project description:Transcriptional profiling of cotyledon transcriptomics at the seedling stage (6 d) by comparison of wild-type vs. cotyledon-less laterne (= pid enp) homozygous mutant. The goal was to determine the transcriptomic profile of a cotyledon. The experiment took advantage of the endogenously caused lack of cotyledons instead of dissecting these organs, which would cause wound-induced expression.This was achieved by comparing seedlings of the Arabidopsis thaliana pid enp double mutant, which is incapable to generate cotyledons. This is caused by the loss of apical cell polarisation of the auxin efflux carrier PIN1 in epidermal cells during embryogenesis.