Project description:This SuperSeries is composed of the following subset Series: GSE27187: Expression profile of acute monocytic leukemia patients GSE27193: Normalization of whole-genome SNP data from acute monocytic leukemia patients for exome sequencing Refer to individual Series
Project description:To understand the pathogenesis of DNMT3A in acute monocytic leukemia (AML-M5), we identified genes that are expressed differently in leukemia cells from AML-M5 patients collected at diagnosis with DNMT3A mutations (6 cases) compared to those without the mutations (4 cases). Differences of expression level were observed in 889 out of 20,723 (4.3%) annotated genes by using Affymetrix microarray with 469 genes upregulated and 420 genes downregulated. Leukemia cells in bone marrow of acute monocytic leukemia patients were collected at diagnosis for RNA extraction and hybridization on Affymetrix microarrays. 6 cases of AML-M5 samples with DNMT3A mutations and 4 cases of AML-M5 samples wihtout DNMT3A mutations were used.
Project description:The experiment aimed to characterize the extent of genomic amplifications in Acute Myeloid Leukemia patients with 8q24 copy number gain, by combining SNP array with whole genome next generation sequencing by Illumina Xten platform