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Triticum aestivum

ABSTRACT: Genome-wide sequencing small RNAs in response to powdery mildew and heat stress in wheat

OTHER RELATED OMICS DATASETS IN: GSE27339

PROVIDER: PRJNA142095 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR101427.fastq.gz	Fastqsanger.gz
	SRR101428.fastq.gz	Fastqsanger.gz
	SRR101429.fastq.gz	Fastqsanger.gz
	SRR101430.fastq.gz	Fastqsanger.gz
	SRR101431.fastq.gz	Fastqsanger.gz

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Genome-wide sequencing small RNAs in response to powdery mildew and heat stress in wheat

Project description:To test whether non-coding RNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing and computational analysis and experimental approach we cloned the small RNAs and identified 125 putative long npcRNAs from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. Among long non-coding RNAs, some were precursors of small RNAs such as microRNAs and siRNAs, two long npcRNAs were identified as signal recognition particle (SRP) 7S RNA variants, and three were characterized as U3 snoRNAs. Wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress.

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Genome-wide sequencing small RNAs in response to powdery mildew and heat stress in wheat

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Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection

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Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection

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Triticum aestivum strain:HD2985, HD2329 Transcriptome or Gene expression

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Microarray analysis of heat and/or drought sensitivity of early grain development in wheat (Triticum aestivum L.)

Project description:Based on EST-based in silico gene expression analysis a 15k oligonucleotid microarray has been developped in order to monitor environmental stress-dependent gene expression changes in the wheat caryopsis. Using this array, the effect of water withdrawal, with and and without additional heat stress, during the first five days of grain development (0-5 DAA) has been investigated on two wheat cultivars differing in their drought sensitivity. The combined effect of heat and drought (DH) on gene expression was much significant (8-10% of the investigated genes changed >2-fold) in contrast to drought alone (1.5%). Drought and heat stress resulted in the co-ordinated change of the expression of storage proteins, some enzymes involved in sugar/starch metabolism, cell division-related and histone proteins, certain transcription factors, heat shock proteins, proteases and aquaporins. The potential link between the observed gene expression changes and the parallel histological observations indicating the accelerated development of the stressed grains is discussed.

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Expression data from Triticum aestivum L. roots

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