Project description:We report the application of the Illumina/Solexa sequencing-by-synthesis platform to identify microRNA, which invovled in bat hibernating regualtion, in brain and adipose tissue. Total 866 microRNAs (including 477 novels) were identified and 269 microRNAs showed significant expression differences during hibernation, including 183 in brain and 199 in adipose tissue. Expressed pattern analysis indicated that two-thirds of differential expressed microRNAs showed down-regulated during hibernation in both tissues. This study will provide clules to in-deep analysis the molecular mechanism of hibernating regualtion. Identification of microRNA on hibernating regulation in 2 different tissues and 2 physiological states of bat.
Project description:Differential gene expression in a wide range of tissues including brown adipose tissue (BAT), liver, heart, hypothalamus, and skeletal muscle in hibernating arctic ground squirrels during multiple stages in torpor-arousal cycles compared to non-hibernating (post-reproductive) animals with illumina beadarray technology. Keywords: Multiple stage comparison
Project description:We report the application of the Illumina/Solexa sequencing-by-synthesis platform to analysis differential expression genes of two key tissues (brain and adipose tissue) during hibernation vs. aroused state, with a hibernating bat species (Myotis ricketti) as model. Total of 12272 genes were identified including 11291 genes in brain and 10691 genes in adipose tissue. Chi-square test indicated that 1943 and 3673 genes in brain and adipose tissues respectively reached the significant level (P ⤠0.01), including 1243 up-regulated and 700 down-regulated genes in hibernating brain and 2621 up-regulated and 1052 down-regulated genes in hibernating adipose tissue. This study provide opportunity to in-deep analysis the molecular mechanism of hibernating regualtion.
Project description:We report the application of the Illumina/Solexa sequencing-by-synthesis platform to identify microRNA, which invovled in bat hibernating regualtion, in brain and adipose tissue. Total 866 microRNAs (including 477 novels) were identified and 269 microRNAs showed significant expression differences during hibernation, including 183 in brain and 199 in adipose tissue. Expressed pattern analysis indicated that two-thirds of differential expressed microRNAs showed down-regulated during hibernation in both tissues. This study will provide clules to in-deep analysis the molecular mechanism of hibernating regualtion.
