Project description:Bmi1 is an important stem cell regulator in multiple tissues, including the lung. We used microarrays to analyze the dysregulation of gene expression in the absence of Bmi1 in the lung, to identify pathways important in Bmi1-dependent lung stem cell regulation Primary lung cells (CD31-negative, CD45-negative) were FACS-isolated from Bmi1 wild-type or mutant mice; RNA was then extracted, amplified, and analyzed using Affymetrix Mouse Genome 430 2.0 Arrays.
Project description:To study effect of VRK1 deletion on spermatogenesis of the mouse, transciptomic analysis of genes in postnatal 8-day testicular cells of wild type and VRK1-deficient Mus musculus was performed. Gene expression in testes from from wild type and VRK1-deficient mutant Mus musculus, respectively, was measured. Four independent experiments for wild type and mutant, respectively, were performed.
