Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA144435 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Stem cell antigen-1 (Sca-1 or Ly6A) is a member of the Ly6 family of glycosyl phostidylinositol (GPI)-anchored cell surface proteins. To determine the potential mechanisms by which Sca-1 regulates cell migration, adhesion, and tumor development; we performed an Affymetrix mouse genome 430A 2.0 array on cDNA comparing shLuc and shSca-1 from cells grown in vitro.
2012-02-16 | GSE30684 | GEO
Project description:Stem cell antigen-1 (Sca-1 or Ly6A) is a member of the Ly6 family of glycosyl phostidylinositol (GPI)-anchored cell surface proteins. To determine the potential mechanisms by which Sca-1 regulates cell migration, adhesion, and tumor development; we performed an Affymetrix mouse genome 430A 2.0 array on cDNA comparing shLuc and shSca-1 from cells grown in vitro. We performed an Affymetrix mouse genome 430A 2.0 array on cDNA comparing shLuc and shSca-1 from cells grown in vitro. Experimental groups were profiled in biological triplicate.
2012-02-16 | E-GEOD-30684 | biostudies-arrayexpress
Project description:Bovine mammary stem cells (MaSC) are a source of ductal and lobulo-alveolar tissue during development of mammary gland and its remodeling in repeating lactation cycles. We hypothesize that the number of MaSC, their molecular properties and interactions with their niche may be essential to determine the mammogenic potential in heifers. To verify this hypothesis we compared the number of MaSC and transcriptomic profile in mammary tissue of 2-year-old, non-pregnant dairy (Holstein-Friesian) and beef (Limousin) heifers. For identification and quantification of putative stem/progenitor cells in mammary tissue sections scanning cytometry was used with a new combination of MaSC molecular markers: stem cell antigen-1 (Sca-1) and fibronectin type III domain containing 3B (FNDC3B) protein. Double labeled cells were located mainly in the basal layers of mammary epithelium. Cytometric analysis of Sca-1pos FNDC3Bpos cells revealed significantly higher number in HF (2.94M-BM-10.35%) than in LM (1.72M-BM-10.20%) heifers. More advanced development of mammary tissue in HF heifers was accompanied by higher expression of intramammary hormones, growth factors, cytokines, chemokines and transcription regulators. The model of transcriptomic niche favorable for MaSC was associated with regulation of genes involved in MaSC maintanence, self renewal, proliferation, migration, differentiation, mammary tissue remodeling, angiogenesis, regulation of adipocyte differentiation, lipid metabolism and steroid and insulin signaling. In conclusion the high mammogenic potential in postpubertal dairy heifers is facilitated by a higher number of MaSC and up-regulation of mammary auto-, paracrine factors representing MaSC niche. Keywords: stem/progenitor cells, transcriptomics, mammary gland, dairy and beef heifers Two-condition experiment, LIM vs. HF. Pulled quarters of mammary glands form 10 LIM heifers (test) and 10 HF heifers (reference). Sample 3 and 4 are dye swaps.
2013-06-16 | E-GEOD-47816 | biostudies-arrayexpress
Project description:∆Np63 regulates stem cell activity in mammary gland development
| PRJNA205535 | ENA
Project description:Bovine mammary stem cells (MaSC) are a source of ductal and lobulo-alveolar tissue during development of mammary gland and its remodeling in repeating lactation cycles. We hypothesize that the number of MaSC, their molecular properties and interactions with their niche may be essential to determine the mammogenic potential in heifers. To verify this hypothesis we compared the number of MaSC and transcriptomic profile in mammary tissue of 2-year-old, non-pregnant dairy (Holstein-Friesian) and beef (Limousin) heifers. For identification and quantification of putative stem/progenitor cells in mammary tissue sections scanning cytometry was used with a new combination of MaSC molecular markers: stem cell antigen-1 (Sca-1) and fibronectin type III domain containing 3B (FNDC3B) protein. Double labeled cells were located mainly in the basal layers of mammary epithelium. Cytometric analysis of Sca-1pos FNDC3Bpos cells revealed significantly higher number in HF (2.94±0.35%) than in LM (1.72±0.20%) heifers. More advanced development of mammary tissue in HF heifers was accompanied by higher expression of intramammary hormones, growth factors, cytokines, chemokines and transcription regulators. The model of transcriptomic niche favorable for MaSC was associated with regulation of genes involved in MaSC maintanence, self renewal, proliferation, migration, differentiation, mammary tissue remodeling, angiogenesis, regulation of adipocyte differentiation, lipid metabolism and steroid and insulin signaling. In conclusion the high mammogenic potential in postpubertal dairy heifers is facilitated by a higher number of MaSC and up-regulation of mammary auto-, paracrine factors representing MaSC niche. Keywords: stem/progenitor cells, transcriptomics, mammary gland, dairy and beef heifers
2013-06-16 | GSE47816 | GEO
Project description:Mammary Tumor Associated RNAs impact tumor cell proliferation, invasion and migration
| PRJNA295152 | ENA
Project description:Stem cells antigen-1 (Sca-1) is an 18-kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein that has widely been used for isolation of murine hematopoietic stem cells (HSCs). However, recently several studies have reported the expression of this protein in non-HSCs such as the liver, skin and muscles among others. In the current study, we used fluorescence-activated cell sorting (FACS) technique to sort Sca-1+ stem cells from mouse hindlimb muscles. The Sca-1+ cells were divided into two sets first set of Sca-1+ cells were processed for protein extraction immediately after sorting (ex vivo set) while the second set of samples, referred to as in vitro set, were expanded in cell culture after sorting and harvested after two passage and then processed for protein extraction. A high-resolution proteome map of the ex vivo and in vitro sets was generated Orbitrap Fusion Tribrid Mass Spectrometer. In total, the analysis led to the identification of 5,581 protein groups.
2021-06-21 | PXD022247 | Pride
Project description:The gut microbiome has garnered attention as an effective target to boost immunity and improve cancer immunotherapy. We found that B cell defective (BCD) mice, such as µ membrane targeted deletion (µMT) and activation-induced cytidine deaminase (AID) knockouts (KO), have elevated anti-tumor immunity under specific-pathogen-free but not germ-free conditions. Microbial dysbiosis in these BCD mice enriched the type I interferon (IFN) signature in mucosal CD8+ T cells, resulting in upregulation of the type I IFN-inducible protein stem cells antigen-1 (Sca-1). Among CD8+ T cells, naïve cells predominantly circulate from the gut to periphery, and those that had migrated from the mLN to the periphery had significantly higher expression of Sca-1. The gut-educated Sca-1+ naïve subset is endowed with enhanced mitochondrial activity and anti-tumor effector potential. The heterogeneity and functional versatility of the systemic naïve CD8+ T cell compartment was revealed by single cell analysis and functional assays of CD8+ T cell subpopulations. These results indicate one of the potential mechanisms through which microbial dysbiosis regulates anti-tumor immunity.
2023-08-03 | GSE155632 | GEO
Project description:The aim of our study was to evaluate gene expression of canine mammary cancer stem-like cells co-cultured with tumor associated macrophages. Two canine mammary cancer cell lines (CMT-U27 and P114) were stained using anti-Sca1 (stem cell antigen 1), anti-EpCAM (Epithelial cell adhesion molecule) and anti-CD44 antibody. The FACS analysis showed 0,02-0,05% of Sca1+/EpCAM+/CD44+ in each of the cell line. Cancer stem-like cells were collected using FACS Aria II then co-cultured with tumor associated macrophages and used for further analysis of gene expression ( using Agilent Gene Expression Hybridization Kit ). Canine mammary cancer cell lines were stained using anti-Sca1 (stem cell antigen 1), anti-EpCAM (Epithelial cell adhesion molecule) and anti-CD44 antibodies. Next using FACS Aria II and Sca1+/EpCAM+/CD44+ cells were collected and co-cultured with tumor associated macrophages. Then, total RNA was isolated and hybridized at Gene Expression microarray.
2016-06-01 | E-GEOD-73230 | biostudies-arrayexpress
Project description:This model describes the humoral and cellular response of the immune system to a tumor associate antigen and the recognition process between B cells, T cells and antigen presenting cells. The model is used to analyze and investigate the competition between immune system cells and mammary carcinoma cells under the action of a vaccine.
2019-11-13 | MODEL1907260002 | BioModels