Project description:Gene expression pattern of Arabidopsis (WT and wrky6) colonized with Piriformospora indica under low and normal phosphate conditions
Project description:Transcriptional profiling of Col4WT and WRKY15 overexpressor (WRKY15OE) plants, grown under controlled and mild salt stress conditions in order to identify molecular mechanisms underlying the increased salt stress sensitivity of WRKY15OE plants.
Project description:au13-08_atpplaii-alpha - transcriptional profilling of arabidopsis plants. Understanding the physiological role of pPLAIIα; under control and drought stress conditions. Study on empty vector, overexpressor and antisense A. thaliana lines.
Project description:The protein kinase OXI1 is induced by a large number of stress conditions and regulates the interaction of plants with pathogenic and beneficial microbes but little is yet known on the underlying mechanisms. In this work, we generated Arabidopsis OXI1 knock out and overexpression lines and show by transcriptome, proteome and metabolome analysis. Our work revealed that OXI1 regulates biosynthesis of SA via CBP60g-induced expression of isochorismate synthase SID2. OXI1 also induces the transcription factor WRKY33 and its downstream target PAD3, resulting in accumulation of camalexin. Moreover, OXI1 regulates ALD1, SARD4 and FMO1 to promote the biosynthesis of pipecolic acid (Pip) and N-hydroxy pipecolic acid (NHP), mediating systemic acquired resistance to bacterial infection. In contrast to OXI1 overexpressor plants, OXI1 knock out plants show enhanced expression of nuclear and chloroplast genes of photosynthesis, and accordingly enhanced growth under ambient conditions. Overall, these results show that OXI1 plays a key role in regulating the trade-off between growth and defense in plants.
Project description:This study describes physiological changes, morphological adaptations and the regulation of pathogen defense responses in Arabidopsis crown galls. Crown gall development was induced on intact plants under most natural conditions with Agrobacterium tumefaciens. Differential gene expression and the metabolite pattern was determined by comparing crown galls with mock-inoculated inflorescence stalk segments of the same age.
Project description:Transcriptional profiling of Arabidopsis thaliana 12-days old seedlings comparing Col-0 wild type with transgenic plants with altered expression of dual-targetting plastid/mitochondrial organellar RNA-polymerase RPOTmp. Transgenic plants used for experiment were: overexpressor plants obtained by transformation of Col-0 WT plants with genetic constructs created in [Tarasenko et al., 2016] contained catalytic part of RPOTmp enzyme with transit peptides of RPOTm (mitochondrial) and RPOTp (plastid) by agrobacterial transformation; plants with complementation of RPOTmp functions in mitochondria or chloroplasts obtained from transformation of GABI_286E07 rpotmp knockout-mutant plants with genetic constructs created in [Tarasenko et al., 2016]. Goal was to determine the effects of RPOTmp knockout/overexpression on global Arabidopsis thaliana gene expression.
Project description:Genome-wide identification of mis-spliced/intron-retained transcripts in Arabidopsis thaliana rcf1-1 mutant plants under normal growth conditions as determined by tiling array analysis.
