Project description:Soybean fast neutron mutant lines were maintained to an advanced generation (ranging between approximately M6 and M11) and compared to their wild-type parent (M92-220-Long) using CGH to identify sequence deletions and duplications in the mutant plants.
Project description:One short trichome mutant (family known by multiple names, including R59C46, FN0175946, and FNMN0409) was identified in a soybean fast neutron population (known as VP02 in Bolon et al. 2011). An advanced line of this mutant was compared to its wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with the short trichome phenotype.
Project description:Ten mutants with altered seed composition traits were identified in a soybean fast neutron population (Bolon et al. 2014). These mutant lines were maintained to an advanced generation (ranging between M5 and M9) and compared to their wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with the seed composition changes.
Project description:Two high-sucrose/low-oil mutants (FN0176450/2012CM7F040P06/SRX826343 and FN0176450/2012CM7F040P05/SRX826351) were identified in a soybean fast neutron population (Bolon et al. 2014). These mutant lines were then advanced to the M3:7 generation and compared to thier wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with high-sucrose/low-oil.
Project description:Four seed composition mutants (known as G15FN-109-1, G15FN-12-1, G15FN-23-3, and G15FN-54-3) were identified in a soybean fast neutron population developed in southern U.S. lines. The parent line for G15FN-109-1 is G00-3880, while the parent line for the other three mutants is G00-3213. Each mutant was compared to its respective parent line using CGH to identify the causative region/gene associated with the seed composition phenotypes.
Project description:CGH was used to compare induced structural variation among soybean fast neutron lines, developed in background M92-220. 114 different soybean fast neutron lines were hybridized against the parental reference genotype M92-220. The soybean tiling array consists of 700k probes, spaced at approximately 1.1 kb intervals.
Project description:CGH was used to compare induced structural variation among soybean fast neutron lines, developed in background M92-220. 11 different soybean fast neutron lines were hybridized against the parental reference genotype M92-220. The soybean tiling array consists of 700k probes, spaced at approximately 1.1 kb intervals.
