Project description:ra10-03_eliciteur - oligosaccharide treatment - 24 hpt - Do the treatments modify gene expression in a way that can give us a hint as to their use in crop protection? What is the effect of the adjuvant on gene expression? - 6,5-week-old arabidopsis plants grown in short-day photoperiod were spray-treated with water, an adjuvant or oligosaccharides in the adjuvant (10 plants per condition, 4 replicates per condition). 6 hrs, 24 hrs and 48 hrs after the treatment, samples were taken (2 7mm diameter leaf disks per plant, from medium-aged leaves), frozen in liquid nitrogen, and later on total RNA was extracted.
Project description:ra10-03_eliciteur - oligosaccharide treatment - 24 hpt - Do the treatments modify gene expression in a way that can give us a hint as to their use in crop protection? What is the effect of the adjuvant on gene expression? - 6,5-week-old arabidopsis plants grown in short-day photoperiod were spray-treated with water, an adjuvant or oligosaccharides in the adjuvant (10 plants per condition, 4 replicates per condition). 6 hrs, 24 hrs and 48 hrs after the treatment, samples were taken (2 7mm diameter leaf disks per plant, from medium-aged leaves), frozen in liquid nitrogen, and later on total RNA was extracted. 16 dye-swap - treated vs untreated comparison
Project description:The phytopathological studies often deal with approaches involving the employment of high elicitor concentrations necessary to provoke responses that could be easily observed and quantified. We tested the range of gene expression changes for a low concentration chitosan elicitor treatment of seedlings, 0.001%, and we compared it with the higher one, 0.01%. We uncover both the quantitative and qualitative changes in gene expressions between the two treatments. Further on, we tested if the gene expression can be modified by the treatment with the defence-related CAPE peptide (10 uM). We discovered several interesting candidate genes specific for each of treatments, and combination of treatments, that would be worth of further more focused genetic studies.
Project description:Transcriptional profiling of SA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response
Project description:Transcriptional profiling of MeJA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response
