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Magallana gigas

ABSTRACT: RNA-seq of Pacific oyster Crassostrea gigas under different developmental stages and stress treatments

PROVIDER: PRJNA146329 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR334212_1.fastq.gz	Fastqsanger.gz
	SRR334212_2.fastq.gz	Fastqsanger.gz
	SRR334213_1.fastq.gz	Fastqsanger.gz
	SRR334213_2.fastq.gz	Fastqsanger.gz
	SRR334214_1.fastq.gz	Fastqsanger.gz

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Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia

Project description:Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O2 L-1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. This is the first study employing microarrays to characterize the genetic markers and metabolic pathways responding to hypoxic stress in C. gigas.

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Project description:Transcriptome sequencing of Pacific oyster Crassostrea gigas

| PRJNA154617 | ENA

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Project description:Deep sequencing of samples from different development stages, different adult organs and different stress treatments of Pacific oyster Crassostrea gigas Samples of 38 developmental stages from egg to juvenile were analyzed using single-end 49 bp RNA-seq. Two libraries mixed by RNAs from different developmental stages were analyzed using paired-end 90 bp RNA-seq. A total of 11 samples mainly from 8 organs (mantle, gill,adductor muscle, digestive gland, hemocyte, labial palp, female gonad and male gonad) were analyzed using paired-end 90 bp RNA-seq. At the same time, single-end 49 bp RNA-seq was conducted on 61 samples collected from adult oysters subjected to 9 types of environmental stressors (exposure to air, salinity, temperature, and exposure to metals).

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Project description:Oyster Crassostrea gigas Transcriptome

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