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Homo sapiens

ABSTRACT: Human bronchial epithelial cells exposed to A. alternata spores

OTHER RELATED OMICS DATASETS IN: E-GEOD-32893

PROVIDER: PRJNA146633 | ENA |

REPOSITORIES: ENA

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Human bronchial epithelial cells exposed to A. alternata spores

Project description:This present study is the first to investigate the global changes in host gene expression during the interaction of human bronchial epithelial cells and live Alternaria spores. Human bronchial epithelial cells (BEAS2-B) were exposed to spores or media alone for 24 hours. RNA was collected from three biological replicates/treatment and used to assess changes in gene expression patterns using Affymetrix Human Genome U133 Plus 2.0 Arrays. Interestingly, many cytokine/chemokine immune response genes were upregulated. Genes involved in cell death, retinoic acid signaling, TLR3, and interferon response pathways were also significantly upregulated. Three biological replicates for control (untreated cells) and treatment (5 x 10^5 A. alternata spores)

2011-12-01 | E-GEOD-32893 | biostudies-arrayexpress

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Human bronchial epithelial cells exposed to A. alternata spores

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Project description:We compared the gene expression stimulated with fungal extracts from Aspergillus (A.) fumigatus, Alternaria (A.) alternata, or Penicillium (P.) notatum in NCI-H292 (a human bronchial epithelial cell line) to search Allergic bronchopulmonary mycosis (ABPM)-related genes. We identified a mucin-related MUC5AC gene, the expression of which was selectively induced by A. fumigatus. Total RNA from NCI-H292 cells stimulated for 24 h with the A. fumigatus, A. alternata, or P. notatum fungi extracts was extracted and subjected to microarray analysis. Each experiments were perfomed once for each stimulus.

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