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Rhizoctonia solani AG-1 IA

ABSTRACT: Evolution and Pathogenic Mechanism of Rice Sheath Blight Pathogen

PROVIDER: PRJNA147097 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR350922_1.fastq.gz	Fastqsanger.gz
	SRR350922_2.fastq.gz	Fastqsanger.gz
	SRR350923_1.fastq.gz	Fastqsanger.gz
	SRR350923_2.fastq.gz	Fastqsanger.gz
	SRR350924_1.fastq.gz	Fastqsanger.gz

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Rhizoctonia solani AG-1 IA strain:AG1 IA

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Complete Nucleotide Sequence of a Partitivirus from Rhizoctonia solani AG-1 IA Strain C24.

Project description:The complete genome of a novel double-stranded (ds) RNA mycovirus, named as Rhizoctonia solani partitivirus 5 (RsPV5), isolated from rice sheath blight fungus R. solani AG-1 IA strain C24, was sequenced and analysed. RsPV5 consists of two segments, dsRNA-1 (1899 nucleotides) and dsRNA-2 (1787 nucleotides). DsRNA-1 has an open reading frame (ORF) 1 that potentially codes for a protein of 584 amino acid (aa) containing the conserved motifs of a RNA-dependent RNA polymerase (RdRp), and dsRNA-2 also contains a ORF 2, encoding a putative capsid protein (CP) of 513 aa. Phylogenetic analysis revealed that RsPV5 clustered together with six other viruses in an independent clade of the genus Alphapartitivirus, indicating that RsPV5 was a new member of the genus Alphapartitivirus, within the family Partitiviridae.

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Rhizoctonia solani AG-1 IA strain:AG1-IA

Project description:Rhizoctonia solani AG-1 IA strain:AG1-IA Transcriptome or Gene expression

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Rhizoctonia solani AG8 infection of wheat seedling roots

Project description:Rhizoctonia solani is a nectrotrophic fungal pathogen that causes billions of dollars of damage to agriculture worldwide and infects a broad host range including wheat, rice, potato and legumes. In this study we identify wheat genes that are differentially expressed in response to the R. solani isolate, AG8-1, using microarray technology. A significant number of wheat genes identified in this screen were involved in ROS production and redox regulation. Levels of ROS species were increased in wheat root tissue following R. solani infection as determined by NBT, DAB and titanium sulphate measurements/stainings. Pathogen/ROS related genes from R. solani were also tested for expression patterns upon wheat infection. TmpL, a R. solani gene homologous to a gene associated with ROS regulation in Alternaria brassicicola, and OAH, a R. solani gene homologous to oxaloacetate acetylhydrolase which has been shown to produce oxalic acid in Sclerotinia sclerotiorum, were highly induced in R.solani when infecting wheat. We speculate that the wheat germin-like protein (GLP) is induced to inactivate the oxalic acid that is produced by the R. solani OAH.

2015-11-04 | GSE74671 | GEO

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