Project description:Male Wistar rats weighing 90-120 g were acclimatized for one week and fed standard laboratory chow, at which time the animals were divided into two groups. Animals were then pair-fed for 8 weeks a regular laboratory chow and water âad libitumâ or Lieber-DeCarli diet (36% calories from ethanol). Control animals received the iso-caloric amount of dextrose to replace ethanol. After 8 weeks of differential feeding rats were euthanized, the pancreas immediately dissected and stored at -80?C until RNA isolation. RNA expression was analyzed using Affymetrix RAE230A gene chips Experiment Overall Design: pancreas from 3 rats feed control diets and 3 rats feed ethanol diets were analyzed
Project description:Gene expression profile in pancreatic islets from control rats fed a standard chow diet and obese rats fed a high-caloric cafeteria diet for 30 days.
Project description:Gene expression profile in visceral-pancreatic adipose tissue from control rats fed a standard chow diet and obese rats fed a high-caloric cafeteria diet for 30 days.
