Project description:The pinewood nematode, Bursaphelenchus xylophilus, the pine wilt disease's causal agent, is a migratory endoparasitic nematode skilled to feed on pine tissues and on fungi that colonize the trees. In order to study B. xylophilus secretomes under the stimulus of pine species with different susceptibility to disease, nematodes were exposed to aqueous pine extracts from Pinus pinaster (high susceptible host) and P. pinea (low susceptible host). Sequential windowed acquisition of all theoretical mass spectra (SWATH-MS) was used to determine relative changes in protein amounts between B. xylophilus secretions, and a total of 776 secreted proteins were quantified in both secretomes.
Project description:This study compared the different gene expression of Bursaphelenchus xylophilus in two growth conditions (growing on Botrytis cinerea and inoculating Pinus thunbergii). The goal was to analyze the specifically-expressed genes of the pine wood nematode involved in the early interaction between B. xylophilus and P. thunbergii and screen the pathogenesis related genes of B. xylophilus. Two-condition experiment, Growing on Botrytis cinerea vs. Inoculating Pinus thunbergii . Biological replicates: 3 replicates.
Project description:Significant attention has been garnered in proteomic research on Pinus koraiensis infected by Bursaphelenchus xylophilus. This destructive nematode parasite disrupts the cellular structure of the pine, resulting in wilt and death. The key proteins involved in nematode secretion, cell wall degradation, and host defense responses are currently under investigation.
Project description:This study compared the different gene expression of Bursaphelenchus xylophilus in two growth conditions (growing on Botrytis cinerea and inoculating Pinus thunbergii). The goal was to analyze the specifically-expressed genes of the pine wood nematode involved in the early interaction between B. xylophilus and P. thunbergii and screen the pathogenesis related genes of B. xylophilus.