Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: The Estrogen receptor alpha cistrome defined by DamIP

PROVIDER: PRJNA148121 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR372722.fastq.gz	Fastqsanger.gz
	SRR372723.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Homo sapiens

Project description:Coativator Function Defines the Active Estrogen Receptor Alpha Cistrome

| PRJNA121689 | ENA

Homo sapiens

Project description:Cross-talk between Estrogen Receptor-related Receptor alpha (ERRalpha) and Estrogen Receptor (ERalpha) pathways.

| PRJNA106665 | ENA

Homo sapiens

Project description:Combinatorial regulation of estrogen receptor alpha-responsive promoters

| PRJNA94257 | ENA

Homo sapiens

Project description:Active estrogen receptor-alpha signaling in ovarian cancer

| PRJNA322076 | ENA

Homo sapiens

Project description:An Estrogen Receptor α-bound Human Chromatin Interactome

| PRJNA119301 | ENA

The Estrogen receptor alpha cistrome defined by DamIP

Project description:We have developed a new method to study DNA-protein interaction in vivo called DamIP, which is based on fusing a protein of interest to a mutant form of DNA adenine methylatransferase (Dam) from E. coli. We showed previously that DamIP can efficiently identify in vivo binding sites of Dam-tethered human estrogen receptor alpha (hERα). In current study, we present the cistrome of hERα determined by DamIP and high throughput sequencing (DamIP-seq). The DamIP-seq defined hERα cistrome overlaps significantly with those determined by ChIP-chip or ChIP-seq, but identifies many new binding regions. As shown by conventional ChIP assay, many DamIP-seq unique hERα binding regions show relatively stable hERα binding, whereas DamIP-seq misses some regions with very transient hERα binding. The methyl-adenine modifications introduced by Dam are stable and do not decrease over 12 days. In summary, the current study provides both an alternate view of the hERα cistrome to further understand the mechanism of hERα mediated transcription, and a new tool to explore other transcriptional factors and cofactors. MCF7 cells were transfected with DamK9A or DamK9A-hERalpha. DamIP were performed from each sample and subjected to solexa sequencing

2011-11-19 | E-GEOD-33804 | biostudies-arrayexpress

Homo sapiens

Project description:Estrogen Receptor Alpha Signaling in Cervical Cancer Associated Fibroblasts

| PRJNA273453 | ENA

Homo sapiens

Project description:[E-MTAB-131] Estrogen Receptor alpha estrogen-responsive binding sites in MCF-7

| PRJNA135831 | ENA

Homo sapiens

Project description:Estrogen-responsive Estrogen Receptor alpha binding sites in MCF-7 by ChIP-seq

| PRJNA150381 | ENA

Homo sapiens

Project description:Cooperative interaction between retinoic acid receptor-alpha and estrogen receptor in breast cancer.