Project description:Vesicular stomatitis virus replicates with marked effciency in Ifit2 knockout mouse brains late after infection (6 days after intranasal inoculation), compared to wt brains. Changes in gene expression, especially of interferon-stimulated genes, between late in infection (at 6 d p.i.) compared to early during infection (2 d p.i.) were examined in comparison to wild-type. Total RNA extracted from mouse brains of wt or Ifit2 knockout mice at 6 days after infection, compared to RNA from 2 days after infection.
Project description:Vesicular stomatitis virus replicates with marked effciency in Ifit2 knockout mouse brains late after infection (6 days after intranasal inoculation), compared to wt brains. Changes in gene expression, especially of interferon-stimulated genes, between late in infection (at 6 d p.i.) compared to early during infection (2 d p.i.) were examined in comparison to wild-type.
Project description:Sarm-deficient mice are protected from VSV encephalitis and death. Microarray was done to examine genes contributing to this phenotype WT and KO mice were infected with VSV and brains were harvested at day 5 post-infection for RNA extraction
Project description:To investigate the function of XAF1 in chromatin openning. Wild-type or XAF1 knockout HT29 cells were infected with VSV for 0 or 3 hours,ATAC-seq was performed. Examination of chromatin openness in Wild-type or XAF1 knockout HT29 cells.
Project description:To investigate whether and what miRNAs expression might be regulated by VSV (vesicular stomatitis virus?) challenge, we analyzed the miRNA expression profile of mouse primary peritoneal macrophages infected with VSV by using an array-based miRNA profiling. After the infection of VSV at MOI 10 for 48 h, the array revealed that many miRNAs were up-regulated in macrophages?
Project description:The goal of this study is to compare transcriptome-wide Nm-seq on the poly A+ RNA of wild-type Raw264.7 macrophages to transcriptome-wide Nm-seq on the poly A+ RNA of Raw264.7 macrophages after VSV infection . The Nm-seq profiles of wild-type Raw264.7 poly A+ RNA and VSV infected Raw264.7 poly A+ RNA were generated by deep sequencing using Illumina HiSeq4000 sequencer.
Project description:Human SLK cells were infected with wildtype (wt) and LANA knockout (KO) Kaposi's sarcoma-associated herpesvirus (KSHV), separately for 3 days. Cellular gene expression changes were identified upon the wild type and LANA KO KSHV virus infection compared to the uninfected SLK cells using the human gene expression microarray U133plus2.0.
Project description:Wild type or HIPK2-/- immortalized bone marrow-derived macrophages (iBMDM) were infected with VSV for 6h, followed by whole genome RNA-seq analysis. Conclusions: HIPK2 deficiency aggravates viral infection and inhibited the production of IFNβ.
