Project description:Transcription profiling of murine J1 embryonic stem cells undergoing a differentiation time course to study changes in transcription during stem cell differentiation
Project description:Transcription profiling of mouse embryonic stem cell line CGR8 during differentiation to mesenchymal stem cells and then adipocyte precursors
Project description:Mouse spermatogonial stem cells (SSCs) continuously self-renew on the feeder layers in serum-free culture medium supplemented with glial cell line-derived neurotrophic factor and fibroblast growth factor 2. To identify novel nuclear proteins involved in SSC maintenance, comparative proteomic profiling of nuclear proteins was performed between self-renewing and differentiation-initiated SSCs in culture. The self-renewing SSC cultures were established from C57BL/6 mouse testes. Nuclear fractions from self-renewing SSC cultures treated with ethanol as a vehicle control (spermatogonial stem cells) and differentiation-initiated SSC cultures treated with 0.3 μM retinoic acid for 24 h (spermatogonial progenitor cells) were isolated for proteomic analysis.
Project description:Transcription profiling of mouse embryonic stem cells to identify genes regulated by STAT3 and NANOG, known differentiation inhibitors
Project description:Transcription profiling by high throughput sequencing at four stages of directed cardiac differentiation of mouse embryonic stem cells
