Project description:Pancreatic islets were isolated from Hnf1a (Hepatocyte nuclear factor 1 alpha) knockout and wild-type mice and cultured ex vivo for two days in RPMI medium with 10% FBS before RNA extraction. The transcription profiles were obtained using Affymetrix GeneChip Mouse Genome 430A 2.0 arrays [Mouse430A_2].
Project description:Pancreatic islets were isolated from Hnf1a+/- (Hepatocyte nuclear factor 1 alpha) and wild-type mice and cultured ex vivo for two days in RPMI medium with 10% FBS before RNA extraction. The transcription profiles were obtained using Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].
Project description:Chromatin from mouse hepatocytes was immunoprecipitated with antibodies against Hepatocyte Nuclear Factor 4 alpha (Hnf4a) and IgG. Amplified and labelled ChIP DNA and input DNA were hybridyzed to Mouse PromoterChip 5A.1 arrays.
