Project description:Our goal is to identify Salycilic Acid responsive genes dependent on PLD activation. - The experiment is done on Arabidopsis suspension cells, ecotype Columbia : Research of phospholipase D (PLD) activity implication in the response to SA. The use of primary alcohol, like N-butanol, makes possible to derive PLD activity towards the production of phosphatidylalcohol with the detriment of phosphatidic acid, the product of the PLD. Thus, in the presence of n-butanol, the response of the genes to SA dependent on phosphatidic acid will see their response disturbed. On the contrary, in the presence of tertiary butanol, the response of the genes should not be disturbed, tertiary alcohols not acting on the PLD. Keywords: treated vs untreated comparison
Project description:PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) catalyses the conversion of phosphatidic acid to diacylglycerol. Disruption of two gene encoding this enzyme in Arabidopsis thaliana results in increased phosphatidylcholine synthesis and proliferation of the endoplasmic reticulum (Eastmond et al., [2010] Plant Cell 22: 2796). We performed microarray analysis on two week old wild type Arabidopsis plants and the pah1 pah2 double mutant using the Ath1 chip to determine what effect the double mutation has on global gene expression.
Project description:au10-12_pa - phosphatidic acid in gene expression - Role of DGK in gene expression - Compounds were added 4h before cell harvesting.
Project description:au10-12_pa - phosphatidic acid in gene expression - Role of DGK in gene expression - Compounds were added 4h before cell harvesting. 6 dye-swap - treated vs untreated comparison
