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Strongylocentrotus purpuratus

ABSTRACT: Select microRNAs are essential for early development in the sea urchin.

PROVIDER: PRJNA151931 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR409091.fastq.gz	Fastqsanger.gz
	SRR409092.fastq.gz	Fastqsanger.gz
	SRR409093.fastq.gz	Fastqsanger.gz
	SRR409094.fastq.gz	Fastqsanger.gz
	SRR409095.fastq.gz	Fastqsanger.gz

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Base-resolution DNA methylation maps of purple sea urchin (Strongylocentrotus purpuratus)

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Base-resolution DNA hydroxymethylation maps of purple sea urchin (Strongylocentrotus purpuratus), european lancelet (Branchiostoma lanceolatum) and zebrafish (Danio rerio) using ACE-seq

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Response to elevated pCO2 in Strongylocentrotus purpuratus larvae

Project description:Transcriptional profiling of purple sea urchin (Strongylocentrotus purpuratus) larvae cultured under three different seawater CO2 concentrations 400, 800, 1200 M-BM-5atm. The goal was to determine the effects of CO2, an important climate change variable, on global gene expression Larvae were cultured under three different seawater CO2 concentrations 400, 800, 1200 M-BM-5atm, each with four replicate cultures, and sampled at two developmental stages (gastrula and pluteus)

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Strongylocentrotus purpuratus high resolution developmental transcriptomic time-course

Project description:Classical embryological studies revealed that during mid-embryogenesis vertebrates show similar morphologies. This âphylotypic stageâ has recently received support from transcriptome analyses, which have also detected similar stages in nematodes and arthropods. A conserved stage in these three phyla has led us to ask if all animals pass through a universal definitive stage as a consequence of ancestral constraints on animal development. Previous work has suggested that HOX genes may comprise such a âzootypicâ stage, however this hypothetical stage has hitherto resisted systematic analysis. We have examined the embryonic development of ten different animals each of a fundamentally different phylum, including a segmented worm, a flatworm, a roundworm, a water bear, a fruitfly, a sea urchin, a zebrafish, a sea anemone, a sponge, and a comb jelly. For each species, we collected the embryonic transcriptomes at ~100 different developmental stages and analyzed their gene expression profiles. We found dynamic gene expression across all of the species that is structured in a stage like manner. Strikingly, we found that animal embryology contains two dominant modules of zygotic expression in terms of their protein domain composition: one involving proliferation, and a second involving differentiation. The switch between these two modules involves induction of the zootype; which in addition to homeobox containing genes, also involves Wnt and Notch signaling as well as forkhead domain transcription factors. Our results provide a systematic characterization of animal universality and identify the points of embryological constraints and flexibility. 165 single embryo samples.

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Project description:Gene expression alterations in SoxC knockdown sea urchin embryos

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Single-cell RNA sequencing of the Strongylocentrotus purpuratus larva reveals the blueprint of major cell types and nervous system of a non-chordate deuterostome

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Strongylocentrotus purpuratus

Project description:A high-resolution transcriptome map of the sea urchin embryo

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