Project description:Bacillus subtilis is a Gram-positive bacterium that serves as an important experimental system. B. subtilis NCIB 3610 is an undomesticated strain that exhibits phenotypes lost from the more common domesticated laboratory strains. Here, we announce the complete genome sequence of DK1042, a genetically competent derivative of NCIB 3610.
Project description:In our study, we discovered that AQCA (Anthraquinone-2-carboxylic acid) effectively degrades Bacillus subtilis NCIB 3610 biofilms, a finding of significant research importance. To investigate the underlying mechanisms, we performed transcriptome sequencing of wild-type biofilms treated with either DMSO (control) or AQCA. Statistical analysis of gene expression (P < 0.05) revealed that AQCA treatment led to significant changes: 184 genes were upregulated, while 503 genes were downregulated by at least twofold compared to the DMSO control group.