Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
Project description:ATAC-seq samples from 2 species and 2 cell types were generated to study cis-regulatory element evolution. Briefly, previously generated urinary stem cell derived iPS-cells (Homo sapiens) of 2 human individuals and fibroblast derived cynomolgus macaque iPSCs (Macaca fascicularis) of 2 individuals (Geuder et al. 2021) were differentiated to neural progenitor cells via dual-SMAD inhibition as three-dimensional aggregation culture (Chambers et al. 2009; Ohnuki et al. 2014). The NPC lines were cultured in NPC proliferation medium and passaged 2 - 4 times until they were dissociated and subjected to ATAC-seq together with the respective iPSC clones. ATAC-seq libraries were generated using the Omni-ATAC protocol (Corces et al. 2017) with minor modifications.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs. One-condition experment, gene expression of 3A6
Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.
Project description:Purpose: We performed comparative transcriptome analysis of mesoangioblasts (MABs) derived from different cardiovascular or skeletal muscle tissues to determine possible gene signatures underlying their specific differentation capacities and define future appropriate cell therapy applications. Methods: RNA was extracted from human fetal mesoangioblasts derived from Aorta, Atrium, Ventricle and Skeletal muscle (Ao, A, V and Sk) in proliferative conditions. Deep sequencing was used to determine quantitative gene expression between the different cell populations. Results: MABs derived from different tissues show differences in their transcriptome profile, despite their common ebryologic origin and isolation using common caracterization markers. The expression by Sk MABs of key cardiogenic genes (i.e. Isl1, TBX2) and the identification of active signaling pathways will serve for future skeletal to cardiac differentiation protocols. Conclusion: Cardiogenic Sk MABs may thus represent a easily accessible cell source for cardiac regeneration.