Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA153345 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR438679_1.fastq.gz | Fastqsanger.gz | |||
| SRR438679_2.fastq.gz | Fastqsanger.gz | |||
| SRR438680_1.fastq.gz | Fastqsanger.gz | |||
| SRR438680_2.fastq.gz | Fastqsanger.gz | |||
| SRR438681_1.fastq.gz | Fastqsanger.gz |
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Project description:Drosophila melanogaster is a well-studied genetic model organism with several large-scale transcriptome resources. Here we investigate 7,952 proteins during the fly life cycle from embryo to adult and also provide a high-resolution temporal time course proteome of 5,458 proteins during embryogenesis. We use our large scale data set to compare transcript/protein expression, uncovering examples of extreme differences between mRNA and protein abundance. In the embryogenesis proteome, the time delay in protein synthesis after transcript expression was determined. For some proteins, including the transcription factor lola, we monitor isoform specific expression levels during early fly development. Furthermore, we obtained firm evidence of 268 small proteins, which are hard to predict by bioinformatics. We observe peptides originating from non-coding regions of the genome and identified Cyp9f3psi as a protein-coding gene. As a powerful resource to the community, we additionally created an interactive web interface (http://www.butterlab.org) advancing the access to our data.
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Project description:Tissue-specific ChIP-seq for RNA pol II and H2A.v in Drosophila melanogaster
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