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Bacillus anthracis

ABSTRACT: Strand-specific RNA-seq reveals ordered patterns of sense and antisense transcription in Bacillus anthracis

PROVIDER: PRJNA153455 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR445246.fastq.gz	Fastqsanger.gz
	SRR445247.fastq.gz	Fastqsanger.gz
	SRR445248.fastq.gz	Fastqsanger.gz
	SRR445249.fastq.gz	Fastqsanger.gz
	SRR445250.fastq.gz	Fastqsanger.gz

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Similar Datasets

Strand-specific RNA-seq reveals ordered patterns of sense and antisense transcription in Bacillus anthracis

Project description:Although genome-wide transcriptional analysis has been used for many years to study bacterial gene expression, many aspects of the bacterial transcriptome remain undefined. A prominent example is antisense transcription, which has been observed in a number of bacteria, though the function of antisense transcripts, and their distribution across the bacterial genome, is still unclear. Single-stranded RNA-seq results revealed a widespread and non-random pattern of antisense transcription covering more than two-thirds of the B. anthracis genome. Our analysis revealed a variety of antisense structural patterns, suggesting multiple mechanisms of antisense transcription. The data revealed several instances of sense and antisense expression changes in different growth conditions, suggesting that antisense transcription may play a role in the ways in which B. anthracis responds to its environment. Significantly, genome-wide antisense expression occurred at consistently higher levels on the lagging strand, while the leading strand showed very little antisense activity. Intrasample gene expression comparisons revealed a gene dosage effect in all growth conditions, where genes farthest from the origin showed the lowest overall range of expression for both sense and antisense directed transcription. Additionally, transcription from both strands was verified using a novel strand-specific assay. The variety of structural patterns we observed in antisense transcription suggests multiple mechanisms for this phenomenon, indicating that some antisense transcription may play a role in regulating the expression of key genes, while some may be due to chromosome replication dynamics and transcriptional noise.

2012-03-15 | GSE36506 | GEO

Strand-specific RNA-seq reveals ordered patterns of sense and antisense transcription in Bacillus anthracis

2012-03-15 | E-GEOD-36506 | biostudies-arrayexpress

Bacillus anthracis Stern 34F2 sporulation

Project description:Timecourse from log phase growth to sporulation in Bacillus anthracis Stern 34F2 Keywords: time-course

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Characterization of BatIM, the cytosine methyltransferase of Bacillus anthracis

Project description:5-methylcytosine is one of the major epigenetic modifications of DNA in living organisms. In bacteria, some species possess DNA methyltransferases that produce the cytosine modification in both strands or either strand of its target sequence. The purpose of this study is to characterize BatIM, the orphan cytosine methyltransferase coded on a prophage region of Bacillus anthracis.

2022-03-02 | GSE160371 | GEO

Direct regulons of the master virulence regulator AtxA of Bacillus anthracis

Project description:AtxA, the master virulence regulator of Bacillus anthracis, regulates the expression of three toxins that are required for the pathogenicity of Bacillus anthracis. Recent transcriptome analyses also showed that AtxA affects a large number of genes on both chromosome and plasmid, suggesting its role as a global regulator. Its mechanism of gene regulation nor binding target in vivo was, however, not well understood. In this work, we conducted ChIP-seq for cataloging binding sites of AtxA in vivo and Cappable-seq for catalogging the transcription start sites on the B. anthracis genome. For detected regulons, single knockout strains were constructed and RNA-seq was conducted for each strain.

2021-06-28 | GSE167871 | GEO

Bacillus anthracis Stern 34F2 sporulation

Project description:Timecourse from log phase growth to sporulation in Bacillus anthracis Stern 34F2

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Comparative Genomics of Bacillus cereus and Bacillus anthracis

Project description:The aim of the study was to carry out a CGH study utilizing a set of 39 diverse Bacillus isolates. Thirty four B. cereus and five B. anthracis strains and isolates were chosen so as to represent different lineages based on previous characterizations, including MLEE and MLST (Helgason, Okstad et al. 2000; Helgason, Tourasse et al. 2004). They represent the spectrum of B. cereus phenotypic diversity by including soil, dairy and periodontal isolates in addition to virulent B. anthracis strains.

2009-11-25 | GSE19068 | GEO

Bacillus anthracis gene expression upon plantazolicin and Me2-Arg-Az5

Project description:Subinhibitory concentrations of compound used to assess their influence on the gene expression profile of Bacillus anthracis.

2017-02-28 | GSE73343 | GEO

Expression analysis of Bacillus anthracis Sterne wild-type and deltaClpX mutant strains

Project description:Investigation of whole genome expression level changes in Bacillus anthracis Sterne deltaClpX mutant compared to the wild-type strain after growth in nutrient rich media. The deltaClpX mutant used in this study is described in McGillivray et al. 2009. ClpX Protease Contributes to Antimicrobial Peptide Resistance and Virulence Phenotypes of Bacillus anthracis. Journal of Innate Immunity 1(5): 494-506.

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Project description:We have analyzed the variation of transcriptome of HUVECs intoxicated by the lethal toxin of Bacillus anthracis at 4 and 8 hours

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