ABSTRACT: Microarray analysis on spleens and livers of mice inoculated subcutaneously with J558 myeloma cells and supplemented with oil palm phenolics (OPP)
Project description:This SuperSeries is composed of the following subset Series: GSE30905: Microarray analysis on livers, spleens and hearts of mice given distilled water fed with either a normal diet or an atherogenic diet GSE30907: Microarray analysis on livers, spleens and hearts of mice fed an atherogenic diet and supplemented with either oil palm phenolics (OPP) or distilled water Refer to individual Series
Project description:OPP (1500 ppm gallic acid equivalent (GAE)) was supplemented to BALB/c mice inoculated subcutaneously with J558 myeloma cells at the dorsum of the neck in order to observe possible immune responses in cancer suppression. Spleens and livers were harvested at three designated time points, eighteen hours, one week and four weeks after inoculation of the cells for gene expression studies. Results show that the expression of immune-related genes in the spleen was delayed, while those related to inflammation in the liver were down-regulated across time, thus suggesting that OPP may have anti-inflammatory properties in suppressing cancer.
Project description:OPP (1500 ppm gallic acid equivalent (GAE)) was supplemented to BALB/c mice inoculated subcutaneously with J558 myeloma cells at the dorsum of the neck in order to observe possible immune responses in cancer suppression. Spleens and livers were harvested at three designated time points, eighteen hours, one week and four weeks after inoculation of the cells for gene expression studies. Results show that the expression of immune-related genes in the spleen was delayed, while those related to inflammation in the liver were down-regulated across time, thus suggesting that OPP may have anti-inflammatory properties in suppressing cancer. Total RNA obtained from spleens and livers of BALB/c mice given OPP (four weeks after subcutaneous inoculation of J558 myeloma cells) were compared to controls given distilled water at three time points, eighteen hours, one week and four weeks after inoculation of the cells (three replicates per group per time point)
Project description:OPP (1500 ppm gallic acid equivalent (GAE)) was supplemented to BALB/c mice inoculated subcutaneously with J558 myeloma cells at the dorsum of the neck in order to observe possible anti-cancer effects. Tumours which developed were harvested four weeks after inoculation of the cells for gene expression studies. Results show that genes involved in the cell cycle were differentially expressed, and that OPP may have anti-cancer properties in vivo.
Project description:Microarray analysis on livers, spleens and hearts of mice fed an atherogenic diet and supplemented with either oil palm phenolics (OPP) or distilled water
Project description:Microarray analysis on livers, spleens and hearts of mice fed a normal diet and supplemented with either oil palm phenolics (OPP) or distilled water
Project description:OPP (1500 ppm gallic acid equivalent (GAE)) was supplemented to BALB/c mice given a normal diet to observe for possible biological activities. Livers, spleens and hearts were harvested six weeks after the feeding regimen for gene expression studies. Results from the separate microarray data analysis carried out on the different organs show that OPP may have pleiotropic biological properties in vivo.
Project description:OPP (1500 ppm gallic acid equivalent (GAE)) was supplemented to BALB/c mice given an atherogenic diet for six weeks to observe for possible anti-atherogenic effects. The control group received distilled water instead of OPP. Livers, spleens and hearts were harvested six weeks after the feeding regimen for gene expression studies. Results from the separate microarray data analysis carried out on the different organs show that OPP attenuated the effects of the atherogenic diet in the organs.
Project description:OPP (1500 ppm gallic acid equivalent (GAE)) was supplemented to BALB/c mice given an atherogenic diet for six weeks to observe for possible anti-atherogenic effects. The control group received distilled water instead of OPP. Livers, spleens and hearts were harvested six weeks after the feeding regimen for gene expression studies. Results from the separate microarray data analysis carried out on the different organs show that OPP attenuated the effects of the atherogenic diet in the organs. Total RNA obtained from livers, spleens and hearts of BALB/c mice given OPP (six weeks after administration of an atherogenic diet) were compared to controls given distilled water (liver: three replicates in the treatment group versus four replicates in the control group; spleen and heart: three replicates in the treatment group versus three replicates in the control group for both organs)
