Project description:This SuperSeries is composed of the following subset Series: GSE28444: Expression data for MCF-7 treated with PolII inhibitors, Triptolide or alpha-amanitin GSE28502: Expression data for HUVSMC treated with Triptolide GSE28503: Expression data for knockdown of POLRMT or RNA PolII in MCF-7 cell line Refer to individual Series
Project description:Transcription of mRNA in mammalian is mainly performed by RNA polymerase II (PolII). POLRMT is responsible for the production of cytoplasmic and nuclear form of mitochondrial RNA polymerase. The former (mtRNAP) participates in transcription of RNA in the mitochondria while the latter (spRNAP-IV) is responsible for some mRNA transcription in the nucleus. The nature and amount of genes transcribed by spRNAP-IV still remains unclear. Thus, we scanned for possible candidate genes by using Affymetrix. MCF-7 cell line was subjected to siRNA knockdown of either non-targeting, POLRMT, or PolII for 72 hours
