Project description:This SuperSeries is composed of the following subset Series: GSE32633: Jasmonic acid-responsive genes in suspension-cultured rice cells GSE32634: Jasmonic acid-responsive genes in rice leaves Refer to individual Series
Project description:Jasmonic acid (JA) is involved in various developmental processes and defense responses against abiotic and biotic stresses. We identified JA-responsive genes in suspension-cultured rice cells 2-12 h after treatment using rice 44k microarray.
Project description:Jasmonic acid (JA) is involved in various developmental processes and defense responses against abiotic and biotic stresses. We identified JA-responsive genes in suspension-cultured rice cells 2-12 h after treatment using rice 44k microarray. Expression profiling in rice cells treated with JA for 2, 4, 6, 8 and 12 h was compared with that in untreated control using two-color method with two biological replicates.
Project description:This experiment was designed to identify transcribed regions of japonica subspecies of the rice genome. A series of high-density oligonucleotide tiling arrays that represent sense and antisense strands of the entire nonrepetitive sequence of all the 12 chromosomes were designed to measure genome-wide transcription. A total of 12253842 36mer oligonucleotide probes positioned every 46 nt on average were used for this purpose. The probes were synthesized via maskless photolithography at a feature density of approximately 389,000 probes per slide. The arrays were hybridized with fluorescence-labeled cDNA reverse-transcribed from equal amounts of four selected poly(A)+ RNA population (seedling root, seedling shoot, panicle, and suspension cultured cells). Keywords: tiling array, genome-wide transcription
