Project description:A QTL intercross was performed bewteen C57BL/6J and KK/HIL for albuminurea, asthma and cardiovascular related phenotypes. Several QTL were identified for most phenotypes. We performed microarray analysis from liver samples to identify genes differentially expressed between the parental strains. The results helped us narrow down the QTL and identify the candidate genes based on differential expression between the parental strains. At 8 weeks of age, the large lobe of the liver from 3 male and 3 females C57BL/6J and KK/HIJ (12 mice total) was collected. The mice were separated individuallly for 3 days and fasted for 4 hours prior to the tissue collection. The mice were sacrificed by cervically dislocation and perfused with PBS prior to the liver collection.
Project description:Two strains of rodent C57Bl/6J and KK/HIJ were compared to find the most suitable rodent model of obesity and type 2 diabetes. KK/HIJ become larger and glucose intolerant on standard chow but are not as well characterized as the C67Bl/6J model which has a more defined behavioral phenotype. The present study is to asses and determie the most suitable mouse model for our research. We used microarray analysis to examine differences in gene expression in C57Bl/6J and KK/HIJ , male and female adult rodent mice
Project description:To quantify gene expression differences in olfactory epithelium between the mouse (Mus musculus) and the Nile rat (Arvicanthis niloticus), paired-end RNA sequencing (RNA-seq) was used to profile olfactory epithelium transcriptomes of six Nile rats and six mice (C57BL/6J) (one male and one female at the age of 8, 12, and 16 weeks for each species).
Project description:Each expressed gene was tested for differential expression in three mouse tissues (Brain, Kidney, Liver) in a direct comparison of SJL/J vs. C57BL/6J Keywords: direct comparison, multiple tissues Whole RNA from a pool of 5 SJL/J mice was compared to a pool of RNA from C57BL/6J mice with three technical replicates per tissue, no dye swaps.
