Project description:This experiment investigated the transcriptional response of the magnetotactic bacterium Magnetospirillum gryphiswaldense MSR1 to acute copper stress. Mid-exponential-phase cultures were exposed to five milligrams per litre copper for three hours, with untreated cultures sampled at the start and end of the incubation period. Three biological replicates were analysed for each condition. RNA was extracted, sequenced and subjected to quality assessment before reads were mapped to the MSR1 reference genome and gene counts were analysed using DESeq2. The primary comparison assessed copper-exposed cultures against time-matched controls, while the control time comparison provided context for normal growth-associated transcriptional changes.
Project description:Iron is limiting in the environment, bacteria respond to this deprivation by activating genes required for bacterial iron homeostasis. Transcriptional regulation in response to iron in Gram-negative bacteria is largely mediated by the ferric uptake regulator protein Fur, which in the presence of iron binds to a specific sequence in the promoter regions of genes under its control and acts as a repressor. Here we describe comparative global gene expression analysis using DNA microarray based on the whole genome sequence of the magnetotactic bacterium Magnetospirillum magneticum AMB-1 was conducted between wild type strain and a non-magnetic NMA61 mutant strain, generated by mini-Tn5 transposon mutagenesis which is incapable of assimilating iron to cytoplasm. No induction of the fur genes in NMA61 mutant strain was considered to be due to low intracellular iron concentration. In the iron-replete condition, among 4492 genes, 434 genes were down-regulated and 527 genes were up-regulated in the wild type strain. Among 434 genes down-regulated, 299 genes were not down-regulated in NMA61 mutant strain, indicating these genes are candidates of Fur-regulated. Keywords: Iron, magnetotactic bacteria