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Escherichia coli UTI89

ABSTRACT: Uropathogenic strain

PROVIDER: PRJNA16259 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR000868.fastq.gz	Fastqsanger.gz
	SRR000869.fastq.gz	Fastqsanger.gz
	SRR000870.fastq.gz	Fastqsanger.gz
	SRR000871.fastq.gz	Fastqsanger.gz
	SRR000872.fastq.gz	Fastqsanger.gz

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Escherichia coli UTI89

Project description:Conditioning of uropathogenic Escherichia coli to nitrosative stress

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Escherichia coli UTI89

Project description:Uropathogenic E. coli UTI89 cells in the presence of dimethyl sulfoxide or ethanol

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Escherichia coli UTI89 isolate:From pellicle

Project description:Uropathogenic E. coli (HCV) Genome sequencing and assembly

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Uropathogenic E. coli UTI89 cells in the presence of dimethyl sulfoxide or ethanol

Project description:The expression levels of uropathogenic E. coli (UPEC) UTI89 cells in YESCA broth with and without 4% dimehtyl sulfoxide (DMSO) and 2% ethanol (EtOH) were studied.

2012-02-15 | GSE35797 | GEO

Escherichia coli 536

Project description:Uropathogenic strain

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sRNA-mediated regulation of P-fimbriae phase variation in intracellular uropathogenic Escherichia coli

Project description:The experiment design involves RNA-seq of Hfq bound RNA co-immunoprecipitation in the uropathogenic Escherichia coli (UPEC) strain UTI89.

2016-05-31 | E-MTAB-3498 | biostudies-arrayexpress

RNA-seq of E. coli strain UTI89 with isogenic mutants lacking QseB and PmrA following activation with ferric iron

Project description:Antibiotic resistance is a growing global health threat. Most research has focused on understanding how mobile genetic elements are acquired by pathogenic bacteria. However, bacteria have intrinsic chromosomally encoded systems to protect themselves against antimicrobial assault. Our lab has uncovered such a system in uropathogenic E. coli. PmrAB and QseBC are connected two component systems that confer resistance to polymyxins, a last resort antibiotic. The histidine kinase PmrB responds to ferric iron and activates its cognate response regulator PmrA, as well as the non-cognate response regulator QseB. QseC, the other histidine kinase plays an important role in resetting the system. To better understand how PmrAB and QseBC mediate polymyxin resistance, this project aimed to elucidate the regulon of the two response regulators QseB and PmrA in the uropathogenic E. coli strain UTI89. In this strain, isogenic mutants were made lacking qseB and pmrA singly and together. These strains and wild-type UTI89 were stimulated with ferric iron and samples for RNA sequencing were taken prior to stimulation and at 15 and 60 minutes post stimulation. Samples were then sent for sequencing on the Illumina platform and analyzed using Rockhopper software.

2020-10-23 | E-MTAB-9277 | biostudies-arrayexpress

Cell wall-deficient Mycoplasma hominis represses biofilm formation of uropathogenic Escherichia coli.

Project description:The features of Mycoplasma in human organ such lung and urinary tract are enigmatic. Here, the role of M. hominis in regard to biofilm formation of uropathogenic Escherichia coli (UPEC) strain CFT073 was investigated. Although M. hominis were inferred to not impact on UPEC bacterial fitness including growth and productions of signaling molecules as autoinducer-2 (AI-2) and indole, we found that the presence of M. hominis dramatically decreased biofilm formation of UPEC CFT073 as well as slightly repressed attachment and cytotoxicity of that. Importantly, this activity was observed on UPEC strain specifically, not enterohemorrhagic E. coli (EHEC) strain that exists on intestine. Whole-transcriptome profiling and quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed PhoPQ system and anti-termination protein (encoded by ybcQ) participates on the reduction of biofilm formation by M. hominis (corroborated by qRT-PCR). Furthermore, collaborating with previous report that toxin-antitoxin (TA) system involved in biofilm formation, M. hominis increased on the transcriptions of toxin genes including hha (toxin gene in Hha-TomB TA system) and pasT (toxin part in PasT-PasI TA system). Hence, we propose that one possible role of M. hominis is to influence bacterial biofilm formation in urinary tract. Only fourteen genes were induced (2.5-fold) by the presence of M. hominis in Uropathogenic Escherichia coli (UPEC) biofilm cells. Among upregulated genes, ybcQ (encodes anti-termination protein Q homolog) and phoP/phoQ (encode DNA-binding response regulators in two-component regulatory system), were induced by the presence of M. hominis.

2016-01-08 | GSE43376 | GEO

DamX controls reversible cell morphology-switching in uropathogenic Escherichia coli.

Project description:Transcriptional analysis of UTI89 - uropathogenic E.coli (UPEC) strain grown in urine/Luria bertani medium culture in vitro as well as during three distinct phases of UPEC bladder infection: intracellular growth, filament formation and filament reversal. UTI89 was used to infect a bladder epithelial cell line cultured within a dynamic flow chamber system and harvested at particular stages of its pathogenecity cascade. Total RNA was processed and cy3 labeled for microarray analysis using Agilent custom Escherichia coli UTI89 arrays designed using E-Array.

2017-05-28 | E-MTAB-3611 | biostudies-arrayexpress

Uropathogenic E. coli UTI89 cells in the presence of dimethyl sulfoxide or ethanol

Project description:The expression levels of uropathogenic E. coli (UPEC) UTI89 cells in YESCA broth with and without 4% dimehtyl sulfoxide (DMSO) and 2% ethanol (EtOH) were studied. To determine the up- or down-regulated expression profile in the presence of dimethyl sulfoxide (DMSO) and ethanol (EtOH), UPEC UTI89 were grown in YESCA broth with 4% dimethyl sulfoxide (DMSO) or 2% ethanol (EtOH) or no comound at 26°C with 200 rpm shaking for 24 hours were used for RNA extraction and hybridization on Affymetrix microarrays.

2012-02-15 | E-GEOD-35797 | biostudies-arrayexpress

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