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Haemophilus influenzae PittII

ABSTRACT: Isolated from the middle-ear of a child with chronic otitis media

PROVIDER: PRJNA16404 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	AAZI01.dat.gz	Other
	AAZI01.fasta.gz	Fasta.gz
	AAZI01.master.dat	Other
	AAZI01.dat.gz	Other

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Project description:Isolated from the middle-ear of a child with chronic otitis media

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Project description:Isolated from the middle-ear effusion of a child with chronic otitis media

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Haemophilus influenzae PittAA

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Project description:Isolated from the middle ear effusion of child with acute otitis media

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Expression analysis of a full SlrV locus 1 knockout in Haemophilus influenzae strain PittII

2015-06-23 | GSE70172 | GEO

Haemophilus influenzae PittCC

Project description:Isoalted from the middle-ear effusion of a child with chronic otitis media with effusion

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Expression analysis of a full SlrV locus 1 knockout in Haemophilus influenzae strain PittII

Project description:Investigation of whole genome gene expression differences between a full (four-gene) SlrV locus 1 deletion compared to the WT PittII strain. Removal of the four SlrV genes (aka macrophage survival factors : ?msf) results in a competetive defect with the WT strain in in vitro biofilms and in the otitis media chinchilla model. It also results in an inability to persist within macrophages long-term in vitro and to escape from the middle-ear space and infect other sites in the chinchilla model. WT and ?msf strains were grown in a chemically defined media (CDM) to early stationary phase (OD ~1.0). RNA was extracted from two separate cultures (biological replicates). 3-13 probes represent each subcluster(gene) and two replicate probe sets are included on each custom NTHi supragenome chip (technical replicates) thus under each sampling condition 4 separate values are obtained per strain and used for WT:Msf-KO comparisons.

2015-06-23 | E-GEOD-70172 | biostudies-arrayexpress

Haemophilus influenzae PittJJ

Project description:Isoalted from th emiddle ear infusion of a child undergoing treatment for chronic otitis media with effusion

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