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Homo sapiens

ABSTRACT: RNA-seq in breast cancer cell lines after manipulation of miR-23b expression

PROVIDER: PRJNA165157 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR496394_1.fastq.gz	Fastqsanger.gz
	SRR496394_2.fastq.gz	Fastqsanger.gz
	SRR496395_1.fastq.gz	Fastqsanger.gz
	SRR496395_2.fastq.gz	Fastqsanger.gz
	SRR496397_1.fastq.gz	Fastqsanger.gz

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RNA-seq in breast cancer cell lines after manipulation of miR-23b expression

Project description:RNA sequencing technology has been carried out in order to evaluate mRNA expression changes after manipulation of miR-23b in both MCF-7 and MDA-MB-231 breast cancer cell lines Our study implicates miR-23b in cytoskeletal remodeling in breast cancer. To evaluate the entire set of genes modulated by miR-23b we performed RNA-seq after ectopic manipulation of this miRNA in breast cancer cell lines. We over-expressed this miRNA in MCF-7 epithelial cancer cell lines and also reduced its activity by stably transfecting MDA-MB-231 mesenchymal-like cancer cell lines with a specific sponge vector. RNA-seq analysis revealed a number of candidate targets of this miRNA.

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Project description:RNA sequencing technology has been carried out in order to evaluate mRNA expression changes after manipulation of miR-26a in both MCF-7 and MDA-MB-231 breast cancer cell lines. To evaluate the entire set of genes modulated by miR-26a in breast cancer, we performed RNA-seq after ectopic manipulation of this miRNA. We over-expressed miR-26a in MCF-7 epithelial cancer cell lines and also reduced its activity by stably transfecting MDA-MB-231 mesenchymal-like cancer cell lines with a specific sponge vector. GO terms and pathway enriched analysis of the transcripts that significantly change upon miR-26 ectopic manipulation implicates miR-26ab in cell cycle, apoptosis, cell spreading and cell adhesion in breast cancer

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