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Glycine max

ABSTRACT: Methylation Changes in Soybean Early Maturation Seed Compartments Using Laser Capture Microdissection (LCM)

PROVIDER: PRJNA165675 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1290780.fastq.gz	Fastqsanger.gz
	SRR1290781.fastq.gz	Fastqsanger.gz
	SRR1297263.fastq.gz	Fastqsanger.gz
	SRR1297264.fastq.gz	Fastqsanger.gz
	SRR1297265.fastq.gz	Fastqsanger.gz

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Transcript Profiling of Soybean Seed Compartments Using Laser Capture Microdissection Coupled With RNA-Seq

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Transcript Profiling of Soybean Seed Compartments Using Laser Capture Microdissection Coupled With RNA-Seq

Project description:We used laser capture microdissection (LCM) to capture soybean seed compartments and profiled the transcriptomes of several compartments using next-generation sequencing. We profiled the transcriptomes of the embryo-proper and the suspensor region from globular stage embryos and the seed coat parenchyma layer of early maturation stage seeds using the Illumina GAIIx system. Illumina sequencing of transcripts from laser-captured embryo proper and suspensor of globular stage embryo and seed coat parenchyma layer of the early maturation stage seed.

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Lignin deposits in xylem vessels of pedicel regulate water transport during seed maturation in soybean (Glycine max L. Merr.)

Project description:Seed desiccation during maturation is important process for seed post-maturation behavior and harvest. However, the desiccation mechanism in soybean seed maturation is hardly known. In this study, water content in seed, pod and pedicel decreased faster than that in peduncle and stem. Therefore, we focus on the pedicel during seed maturation. By morphological analysis, the deposits in xylem vessels were confirmed in pedicel at 61 day after flowering (DAF), when there are not the deposits in peduncle. It was clarified by microarray analysis that lignin biosynthesis related genes expressed in pedicel at 61 DAF. Indeed, GmPAL, Gm4CL, GmC3H and GmCAD, which are lignin biosynthesis related genes, increased in pedicel during seed maturation. Furthermore, lignin content in pedicel also increased toward at 61 DAF and accumulated in the xylem vessels. These results suggested that lignin deposits into xylem vessels in pedicel cause the soybean seed desiccation during seed maturation.

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Transcription profiling of soybean seeds containing early maturation stage embryos

Project description:We microdissected each compartment from 8-micron paraffin sections using the Leica LMD6000 system to identify all genes active in different compartments of a soybean seed containing a early maturation-stage embryo. Experiment Overall Design: early maturation-stage seed compartments were isolated using the Leica LMD6000 system. Total RNA was amplified and hybridized with Affymetrix Soybean Genome Arrays.

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