Project description:Genome wide DNA methylation profiling of saliva samples from male identical twins discordant for sexual orientation, age 21 to 55. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in bisulfite converted DNA. Samples included 34 pairs of identical twins and one set of identical triplets, and 13 hybridisations were technical replicates of a selection of those samples.
Project description:Genome wide DNA methylation profiling of saliva samples from male identical twins discordant for sexual orientation, age 21 to 55. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in bisulfite converted DNA. Samples included 34 pairs of identical twins and one set of identical triplets, and 13 hybridisations were technical replicates of a selection of those samples. Bisulphite converted DNA isolated from saliva of identical twin pairs were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2
Project description:Genome-wide analysis of DNA methylation, copy number variation, and gene expression in monozygotic twins discordant for primary biliary cirrhosis (PBC). DNA and RNA collection of identical and non identical twins with PBC and performed a genome-wide investigation to determine differences in DNA methylation, CNV, and gene expression.
Project description:We obtained a comprehensive DNA methylation profile of 15 breast cancer discordant twins, using the high resolution Infinium HumanMethylation450 BeadChip platform (450K, Illumina), previously established to reliably detect methylation changes of more than 450,000 CpG sites. To provide insight into the temporal and causal relationships and predictive potential, samples from breast cancer patients before (7) and after diagnosis (8) were also analyzed. Using whole blood from 15 twin pairs discordant for breast cancer and high-resolution (450k) DNA methylation analysis we identified 403 differentially methylated CpG sites including known and novel potential breast cancer genes.
Project description:We obtained a comprehensive DNA methylation profile of 15 breast cancer discordant twins, using the high resolution Infinium HumanMethylation450 BeadChip platform (450K, Illumina), previously established to reliably detect methylation changes of more than 450,000 CpG sites. To provide insight into the temporal and causal relationships and predictive potential, samples from breast cancer patients before (7) and after diagnosis (8) were also analyzed. Using whole blood from 15 twin pairs discordant for breast cancer and high-resolution (450k) DNA methylation analysis we identified 403 differentially methylated CpG sites including known and novel potential breast cancer genes. 30 Samples
Project description:Epigenetic profiling of birth-weight discordant twins using Illumina's 450K Human DNA methylation BeadChip Comparing DNA methylation difference in birth-weight discordant twin pairs
Project description:Genome wide DNA methylation profiling of adipose tissue of MZ twins discordant and concordant for BMI. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 485,000 CpGs. Samples included 24 pairs discordant and 11 pairs concordant for BMI.
