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Mus musculus

ABSTRACT: Fbxl10 regulates PRC1 recruitment to CpG islands and H2A ubiquitination

PROVIDER: PRJNA166917 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR496516.fastq.gz	Fastqsanger.gz
	SRR496517.fastq.gz	Fastqsanger.gz
	SRR496518.fastq.gz	Fastqsanger.gz
	SRR496519.fastq.gz	Fastqsanger.gz
	SRR496520.fastq.gz	Fastqsanger.gz

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Fbxl10 regulates PRC1 recruitment to CpG islands and H2A ubiquitination

Project description:Polycomb repressive complex 1 (PRC1) catalyzes H2A monoubiquitination (uH2A) and regulates pluripotency in embryonic stem cells (ESCs). However the mechanisms controlling PRC1 recruitment and activity are largely unknown. Here we show that Fbxl10 interacts with Ring1B and Nspc1, forming a non-canonical PRC1. We demonstrate that Fbxl10-PRC1 is essential for H2A ubiquitination in mouse ESCs. Genome-wide analyses reveal that Fbxl10 preferentially binds to CpG islands and co-localizes with Ring1B on Polycomb target genes. Notably, Fbxl10 depletion causes modest dissociation of Ring1B but a major loss of uH2A on target genes. Furthermore rescue experiments for Fbxl10 reveal that its DNA binding capability and integration into PRC1 are required for proper H2A ubiquitination. ES cells lacking Fbxl10, like previously characterized Polycomb mutants, show a severely compromised capacity for successful differentiation. Our results shed light on a novel mechanism how CpG islands regulate chromatin function by affecting polycomb recruitment and activity. All ChIP-seq reactions were performed in either untransfected cells, cells expressing scrambled shRNA or Fbxl10 shRNA, Ring1b-/- or Suz12-/- mouse ES cells

2012-12-31 | E-GEOD-37930 | biostudies-arrayexpress

Fbxl10 regulates PRC1 recruitment to CpG islands and H2A ubiquitination

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Project description:KDM2B binds CpG islands and modulates recruitment of Ring1b

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Project description:Fbxl19 recruits Rnf20 to CpG Islands and promotes H2B mono-ubiquitination [Affymetrix]

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Small molecule inhibitor of PRC1 E3 ligase activity induces differentiation of leukemia initiating cells [ChIP-seq]

Project description:We have developed small molecule inhibitors of PRC1. These inhibitors target PRC1, decrease H2A ubiquitination and differentiation of TEX cells.

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Project description:We have developed small molecule inhibitors of PRC1. These inhibitors target PRC1, decrease H2A ubiquitination and differentiation of TEX cells.

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Project description:Fbxl19 recruits Rnf20 to CpG Islands and promotes H2B mono-ubiquitination [ChIP-Seq]

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H2A.B and MeDIP sequence result

Project description:H2A.B is a unique histone H2A variant that shares only 40 ~ 50 % sequence identity with canonical H2A. It has only been identified in mammals and has quickly evolved with remarkable sequence diversity among different species. H2A.B is ubiquitously expressed in most cells and tissues. It is mainly deposited in gene body region. The localization of H2A.B is associated with methylated CpG islands in mouse ES cells. H2A.B facilitates transcription elongation to go through methylated CpG islands in the gene bodies. One typical example is that H2A.B regulates transcription elongation at imprinted loci.

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H2ABbd ChIP-seq and MeDIP sequence result

Project description:H2ABbd is a unique histone H2A variant that shares only 40 ~ 50 % sequence identity with canonical H2A. It has only been identified in mammals and has quickly evolved with remarkable sequence diversity among different species. H2ABbd is ubiquitously expressed in most cells and tissues. It is mainly deposited in gene body region.The localization of H2ABbd is associated with methylated CpG islands in mouse ES cells.H2ABbd facilitates transcription elongation to go through methylated CpG islands in the gene bodies. One typical example is that H2ABbd regulates transcription elongation at imprinted loci.

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H2A.B and MeDIP sequence result

2013-12-24 | E-GEOD-48892 | biostudies-arrayexpress