Aeromonas hydrophila subsp. hydrophila ATCC 7966
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA16697 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Aquatic organism that can cause foodborne illnesses in humans
| PRJNA35883 | ENA
Project description:Aeromonas hydrophila ATCC 7966 DAP-Seq epigenomics
| PRJNA757860 | ENA
Project description:Aeromonas hydrophila strain:ATCC 7966 Genome sequencing and assembly
| PRJNA980543 | ENA
Project description:Aeromonas hydrophila strain:ATCC 7966 Transcriptome or Gene expression
| PRJNA345515 | ENA
Project description:Aeromonas hydrophila Transcriptome
| PRJNA345514 | ENA
Project description:The mucosal surfaces of fish serve as the first-line of defense against the myriad of aquatic pathogens present in the aquatic environment. The immune repertoire functioning at these interfaces is still poorly understood. The skin, in particular, must process signals from several fronts, sensing and integrating environmental, nutritional, social, and health cues. Pathogen invasion can disrupt this delicate homeostasis with profound impacts on signaling throughout the organism. Here, we investigated the transcriptional effects of virulent A. hydrophila infection in channel catfish skin, Ictalurus punctatus. We utilized an 8X60K Agilent microarray to examine gene expression profiles at critical early timepoints following challenge—2 h, 8 h, and 12 h. Expression of a total of 2,168 unique genes was significantly perturbed during at least one timepoint. We observed dysregulation of a number of genes involved in antioxidant, cytoskeletal, immune, junctional, and nervous system pathways. In particular, A. hydrophila infection rapidly altered a number potentially critical lectins, chemokines, interleukins, and other mucosal factors in a manner predicted to enhance its ability to adhere and invade the catfish host.
2015-01-01 | GSE40733 | GEO
Project description:Gram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. OMVs have been studied extensively in bacterial pathogens, however, information related with the composition of Aeromonas hydrophila OMVs is missing. In this study we analyzed the composition of purified OMVs from A. hydrophila ATCC® 7966TM by proteomics. Also we studied the effect of OMVs on human peripheral blood mononuclear cells (PBMCs). Vesicles were grown in agar plates and then purified through ultracentrifugation steps. Purified vesicles showed an average diameter of 90-170 nm. Moreover, 211 unique proteins were found in OMVs from A. hydrophila; some of them are well-known as virulence factors such as: haemolysin Ahh1, RtxA toxin, extracellular lipase, HcpA protein, among others. OMVs from A. hydrophila ATCC® 7966TM induced lymphocyte activation and apoptosis in monocytes, as well as over-expression of pro-inflammatory cytokines. This work contributed to the knowledge of the composition of the vesicles of A. hydrophila ATCC® 7966TM and their interaction with the host cell.
| S-EPMC6250952 | biostudies-literature
Project description:The succinylated modification on bacterial protein lysine residues is currently reported to be crucial for regulating cellular physiology and pathology. In this study, to investigate the effect of the lysine succinylation modification on the biological regulation in a well-known fish pathogen, Aeromonas hydrophila, a highly affinity purification was performed to enrich lysine succinylation peptides in A. hydrophila and then identified a total 2174 lysine succinylation sites on 666 proteins with LC-MS/MS. The further motif analysis showed eight motifs surrounding the central lysine succinylated residue were conservative which share some common preferences with other bacterial species. Gene ontology analysis showed that these succinylated proteins involved in diverse metabolic pathways and biological processes, such as translation, protein export and central metabolic pathways. In general, our study provides a significant insight into the functions of lysine succinylation in the cellular physiology and pathology in A.hydrophila.
2018-10-17 | PXD009778 |
Project description:Leading cause of foodborne illnesses worldwide
| PRJNA12516 | ENA
Project description:Fish skin is a critical regulatory organ, serving not only as a physical barrier to pathogen entry, but also as a sophisticated integrator of aquatic environmental, social and nutritional cues through roles in immunity, osmoregulation, and endocrine signaling. Integral to the complexity of teleost skin is the mucus layer secreted by epidermal goblet cells. Pathogen invasion can disrupt this delicate homeostasis with profound impacts on signaling throughout the organism. Here, we investigated the transcriptional effects of virulent A. hydrophila infection in blue catfish skin, Ictalurus furcatus. We utilized an 8X60K Agilent microarray to examine gene expression profiles at critical early timepoints following challenge—2 h, 12 h, and 24 h. Expression of a total of 1,155 unique genes was significantly perturbed during at least one timepoint. We observed dysregulation of a number of genes involved in including antioxidant/apoptosis, cytoskeletal rearrangement, immune response, junctional/adhesion, and proteases. In particular, A. hydrophila infection rapidly altered a number potentially critical lectins, chemokines, interleukins, and other mucosal factors in a manner predicted to enhance its ability to adhere and invade the catfish host.
2014-06-17 | GSE42491 | GEO