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Pseudomonas alcaliphila

ABSTRACT: Pseudomonas alcaliphila overview

PROVIDER: PRJNA167494 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR4234967_1.fastq.gz	Fastqsanger.gz
	SRR4234967_2.fastq.gz	Fastqsanger.gz

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Draft Genome Sequence of Chromate-Resistant and Biofilm-Producing Strain Pseudomonas alcaliphila 34.

Project description:We report the draft genome sequence of Pseudomonas alcaliphila 34, a Cr(VI)-hyperresistant and biofilm-producing bacterium that might be used for the bioremediation of chromate-polluted soils. The genome sequence might be helpful in exploring the mechanisms involved in chromium resistance and biofilm formation.

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Innovative low-cost biosorption process of Cr<sup>6+</sup> by Pseudomonas alcaliphila NEWG-2.

Project description:Chromium is one of the heavy metal pollutants that causing risky health issues when discharged into the aquatic ecosystems. The current investigation focused on the bioremoval of Cr6+ depending on the bacterial sorption process by using Pseudomonas sp. NEWG-2 which was identified on the basis of morphological, cultural characteristics, 16S rRNA sequencing and phylogenetic analysis as Pseudomonas alcaliphila strain NEWG-2. It is clear from the FCCD experiments that the bacterium can grow normally and remove 96.60% of 200 mg/l of Cr6+ using yeast extract (5.6 g/l), glucose (4.9 g/l), pH (7) for 48 h incubation period. SEM and EDS analyses proved that the Cr6+ was biosorbed by P. alcaliphila NEWG-2. FTIR spectra indicated that the phenolic, carbonyl ester, acetyl, carboxylate, alkanes and carbonyl were the main groups involved in the chromium biosorption. Of the equilibrium isotherms models, the Langmuir model was more obedient, with a maximum uptake (qmax) of 10 mg/g (bacterial-alginate beads), than the Freundlich one. The findings reveal the efficiency of P. alcaliphila NEWG-2 in Cr6+ biosorption, with feasibility in the treatment of chromium-contaminated water as a green-technology tool. Interestingly, to the best of our knowledge, this is the first report on Cr6+ biosorption process by P. alcaliphila.

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Complete genome sequence of Pseudomonas alcaliphila JAB1 (=DSM 26533), a versatile degrader of organic pollutants.

Project description:In this study, following its isolation from contaminated soil, the genomic sequence of Pseudomonas alcaliphila strain JAB1 (=DSM 26533), a biphenyl-degrading bacterium, is reported and analyzed in relation to its extensive degradative capabilities. The P. alcaliphila JAB1 genome (GenBank accession no. CP016162) consists of a single 5.34 Mbp-long chromosome with a GC content of 62.5%. Gene function was assigned to 3816 of the 4908 predicted genes. The genome harbors a bph gene cluster, permitting degradation of biphenyl and many congeners of polychlorinated biphenyls (PCBs), a ben gene cluster, enabling benzoate and its derivatives to be degraded, and phe gene cluster, which permits phenol degradation. In addition, P. alcaliphila JAB1 is capable of cometabolically degrading cis-1,2-dichloroethylene (cDCE) when grown on phenol. The strain carries both catechol and protocatechuate branches of the β-ketoadipate pathway, which is used to funnel the pollutants to the central metabolism. Furthermore, we propose that clustering of MALDI-TOF MS spectra with closest phylogenetic relatives should be used when taxonomically classifying the isolated bacterium; this, together with 16S rRNA gene sequence and chemotaxonomic data analyses, enables more precise identification of the culture at the species level.

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