Project description:TIE1 regulates leaf development by repressing leaf differentiation because the semi-dominant mutant tie1-D by activation tagging displays small and hyponastic leaves and the differentiation of leaf epidermal cells is delayed in the tie1-D mutant, whereas disruption of TIE1 causes epinastic leaves with early differentiated epidermal cells. We used microarrays to investigate the molecular base underpinning the phenotypes of TIE1-overexpressing plants. Aerial parts of 14-day-old seedlings from wild-type and GFP-TIE1-15 were collected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:(1) Phosphopeptide abundances of group B-Rafs, SnRK2s or SnRK2 substrates in the aerial parts of soil-grown Arabidopsis wild-type (Col-0) seedlings with/without water supply for indicated periods. Data were obtained from three independent biological replicates. (2)Phosphopeptide abundances in 2-week-old Arabidopsis wild-type (Col-0) seedlings treated with/without dehydration on the filter paper for 30 min. Data were obtained from three independent biological replicates. (3) Phosphopeptide abundances in 2-week-old Arabidopsis wild-type (Col-0), raf13-1raf15-1 or ireh1-2 seedlings under optimal growth conditions. Data were obtained from three independent biological replicates.
Project description:Receptor-like kinases (RLKs) play important roles in plant development and defense responses; however, their functions in other processes remain unclear. Here, we report that OsSIK2, an S-domain RLK from rice, is involved in abiotic stress and senescence process, integrating stress signals into developmental program for adaptive growth. We used microarrays to detail the gene expression in OsSIK2-overexpression and mutant lines from 14-day-old rice seedlings and identified distinct regulated genes. We sought to obtain distinct regulated genes by OsSIK2. To that end,the aerial parts of different rice seedlings,including OsSIK2-overexpression lines, mutant and respective controls, were harveted for total RNA extraction and hybridization on Affymetrix microarrays.
Project description:Gene expression profiles in 15 day old low oil 1 (loo1) mutant and the wild type A15 seedlings were compared. Keywords: Comparison and loo1 and wild type seedling transcriptomes
Project description:Compared analysis of the transcriptomes of 12-day old seedlings from wild type Col-0 treated with NO for 15, 30 and 60 min vs untreated control seedlings. Samples were harvested 12 h after dawn of day 12 after sowing and seedlings were grown under long days (16 h light / 8 h darkness) photoperiodic conditions.
Project description:To understand how GTL1 regulates cell growth, we first identified its potential direct targets by the chromatin immunoprecipitation followed by the hybridization on an Affymetrix Arabidopsis Tiling 1.0R array (ChIP-chip). To enrich the genomic region bound by GTL1 in vivo, we harvested whole aerial parts of 12-day-old gtl1-1 plants complemented with the pGTL:GTL1:GFP constructs and immunoprecipitated the chromatin fragments associated with GTL1-GFP proteins using antibodies against GFP. After applying a cut-off P-values of 0.001of MAT (Model-based analysis of tiling array), we identified a total number of 3,900 putative immediate target genes that showed consistent binding by GTL1.
Project description:Chromatin immunoprecipitation was performed in nlp2-2 Arabidopsis thaliana Col-0 14-d-old seedlings complemented by a pNLP2::NLP2-GFP construct upon 30 minutes NO3- resupply after a 3-day NO3- starvation.
Project description:Transcriptional profiling for wild-type and php mutants Expression of >22,600 genes was analyzed in aerial tissues from 14 day old soil grown wild type and php mutant plants using Affymetrix ATH1 GeneChip, and two independent biological replicates. Data from CEL files were adjusted for background and normalized using the Bioconductor GCRMA package (http://www.bioconductor.org).
