Saccharomyces cerevisiae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA171889 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene deletion expression profiles of yeast chromatin modifiers
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Project description:Compendium of deletion mutant gene expression profiles, 700 responsive mutants [hs1991]
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Project description:In Saccharomyces cerevisiae, specific genes physically relocate from the nucleoplasm to the nuclear periphery concomitant with transcriptional activation, where they associate with the nuclear pore complex (NPC). We took a genomics approach in order to gain insight into the universality and physiological relevance of the interaction between active genes and the NPC. Using synthetic genetic array (SGA) approach, we identified interactions between components of the SAGA histone acetyltransferase complex and the Mlp and Nup60 subunits of the NPC. Cells lacking these SAGA and NPC components display growth defects under optimal growth conditions, in which cells are grown on rich medium containing the preferred sugar glucose as a carbon source and incubated at their optimal temperature. That growth defects were observed under these non-stress conditions suggests that these interactions are indicative of defects in normal cell physiology. These results are consistent with a model where physical interactions between the NPC and SAGA are important for transcription of constitutively expressed genes. To test this hypothesis, we used microarray analysis to assess changes global transcript levels in the absence of Nup60, Ada2, or Nup60 and Ada2. Microarray analysis reveals that the growth defect in these double mutants is correlated with a synthetic reduction in steady-state transcript levels for numerous genes that are strongly expressed in wildtype cells. These results suggest that SAGA and Nup60 cooperate in transcriptional regulation, and are consistent with a model for global regulation of SAGA-dependent transcription at the NPC. Wildype, single deletion, and double deletion cells were inoculated to equal starting ODs in 50 mL YEPD media and grown at 30C with shaking. Cells were collected at mid-log phase, washed with chilled water, and stored at -80C for later processing. RNA was isolated using TRIzol reagent according to the manufacturer's protocol, with modification for yeast cells.
2013-05-13 | E-GEOD-21870 | biostudies-arrayexpress
Project description:The global gene expression pattern by YDR374C deletion
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Project description:Effect of ribosomal protein intron deletion on gene expression
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Project description:HSF1-expression: Molecular mechanisms that distinguish TFIID housekeeping from regulatable SAGA promoters
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Project description:MOT1-expression: Molecular mechanisms that distinguish TFIID housekeeping from regulatable SAGA promoters
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