Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Kluyveromyces waltii genes Total RNA was collected in mid-log phase from Kluyveromyces waltii cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Kluyveromyces waltii.
Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Kluyveromyces lactis genes Total RNA was collected in mid-log phase from Kluyveromyces lactis cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Kluyveromyces lactis.
Project description:A comparison between high and low sulfur source supplies, i.e., sulfate, methionine or cystine, was carried out in order to identify key steps in the biosynthetic and catabolic pathways of the sulfur amino acids. the transcriptomic study was carried out using Agilent 8X15k slides with a custom oligoset designed by V. Loux using the Kluyveromyces lactisORFeome extracted from genolevures database
